Hypoxia/ischemia and the regulation of neuroglobin and cytoglobin expression
Hypoxia/ischemia and the regulation of neuroglobin and cytoglobin expression
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DOI：
10.1080/15216540500037406
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发表时间：
2004-11-01
期刊：
影响因子：
4.6
通讯作者：
Moens, L
中科院分区：
文献类型：
--
作者：
Fordel, E;Geuens, E;Moens, L
与血红蛋白(Hb)和肌红蛋白(Mb)类似，脑红蛋白(Ngb)和细胞球蛋白(Cygb)被认为参与了氧(O-2)的储存和输送。Cygb基因同时含有保守的HREs和mRNA稳定位点，强烈暗示氧依赖的调节。我们用实时定量聚合酶链式反应检测了慢性低氧条件下NGB和Cygb的相对转录变化。我们可以得出结论，Cygb是一个低氧诱导的基因，在慢性低氧过程中，在海马神经元细胞系和多个代谢活跃的组织中转录上调。Cygb的诱导机制依赖于HIF-1α。HIF-1在哺乳动物转录因子中是独一无二的，因为它在低氧诱导下的特异性和敏感性。NGB的表达似乎受其他反应元件的调节，受缺氧的影响较小。
In analogy to hemoglobin (Hb) and myoglobin (Mb), neuroglobin (Ngb) and cytoglobin (Cygb) are supposed to be involved in oxygen (O-2) storage and delivery. The Cygb gene harbours both conserved HREs and mRNA stabilization sites, strongly suggestive of an oxygen-dependent regulation. We examined the relative transcriptional changes of Ngb and Cygb in a situation of chronic hypoxia using real-time quantitative PCR. We could conclude that Cygb is a hypoxia-induced gene, which is transcriptionally upregulated during chronic hypoxia in a hippocampal neuronal cell line and in multiple murine metabolically active tissues. The mechanism of induction of Cygb is HIF-1 alpha dependent. HIF-1 is unique among mammalian transcription factors with respect to the specificity and sensitivity of its induction by hypoxia. Ngb expression seems to be regulated using other response elements and is less influenced by hypoxia.