Engineered neural tissue for peripheral nerve repair
Engineered neural tissue for peripheral nerve repair
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DOI：
10.1016/j.biomaterials.2013.06.025
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发表时间：
2013-10-01
期刊：
影响因子：
14
通讯作者：
Phillips, James B.
中科院分区：
文献类型：
--
作者：
Georgiou, Melanie;Bunting, Stephen C. J.;Phillips, James B.
组织工程技术的新组合为构建排列细胞生物材料提供了一种简单有效的方法。雪旺细胞在系留的1型胶原基质中自我排列，然后去除间质液，产生稳定的组织样生物材料，重建与神经移植物相关的排列的细胞和细胞外基质结构。在共培养模型中，这种工程神经组织片支持和指导神经元生长，初步的体内试验表明，在大鼠坐骨神经修复（5毫米间隙）期间，含有卷曲片棒的装置可以支持神经元生长。进一步的测试表明，在8周后，该装置修复了一个临界尺寸的15毫米间隙，工程神经组织支持了跨间隙的强大的神经元再生。因此，这是产生各向异性工程组织的一种有用的新方法，它可以与雪旺细胞一起制备用于周围神经修复的人工神经组织。(C) 2013 Elsevier Ltd.版权所有。
A new combination of tissue engineering techniques provides a simple and effective method for building aligned cellular biomaterials. Self-alignment of Schwann cells within a tethered type-1 collagen matrix, followed by removal of interstitial fluid produces a stable tissue-like biomaterial that recreates the aligned cellular and extracellular matrix architecture associated with nerve grafts. Sheets of this engineered neural tissue supported and directed neuronal growth in a co-culture model, and initial in vivo tests showed that a device containing rods of rolled-up sheets could support neuronal growth during rat sciatic nerve repair (5 mm gap). Further testing of this device for repair of a critical-sized 15 mm gap showed that, at 8 weeks, engineered neural tissue had supported robust neuronal regeneration across the gap. This is, therefore, a useful new approach for generating anisotropic engineered tissues, and it can be used with Schwann cells to fabricate artificial neural tissue for peripheral nerve repair. (C) 2013 Elsevier Ltd. All rights reserved.