Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb.
Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb.
复制标题
在植物病原真菌大丽黄萎病菌中检测到的新型 dsRNA 病毒的基因组特征。
DOI：
10.1016/j.virusres.2011.04.029
复制
发表时间：
2011-07-01
期刊：
影响因子：
5
通讯作者：
Chen, Ji-shuang
中科院分区：
文献类型：
--
作者：
Cao, Yue-fen;Zhu, Xi-wu;Chen, Ji-shuang
在大丽黄萎病菌 Kleb 中检测到了四个新的双链 RNA 片段。菌株（V. dahliae 分离株 0-21），棉花黄萎病的致病真菌。每个 dsRNA 基因组片段都包含一个大的开放阅读框 (ORF)，编码一种独特的蛋白质，与金病毒属中相应的推定蛋白质具有适度的序列相似性。这些包括RNA依赖性RNA聚合酶(RdRp)、外壳蛋白、未定义的复制相关蛋白和卵巢肿瘤结构域肽酶。对四种假定蛋白的系统发育分析一致表明它们在进化上与金病毒属中的病毒相关。四种 dsRNA 的 5'-和 3'-非翻译区具有高度相似的内部序列，并包含 UGAUAAAAAA(/U)UG(/U)AAAAAA-（在 5'-UTR 中）和 -UUUACUACU（在 3'-UTR 中）的保守序列片段，表明它们具有共同的病毒起源。事实上，从真菌菌丝体中提取了直径约为 34 nm 的等轴病毒样颗粒 (VLP)，并且在病毒样颗粒 (VLP) 部分中也检测到了四个 dsRNA 片段。这些结果表明，来自真菌分离株 0-21 的具有四个不同 dsRNA 基因组片段的真菌病毒是金黄病毒属的新成员。我们将该病毒命名为大丽黄萎病病毒 1 型 (VdCV1)。 (C) 2011 Elsevier B.V. 保留所有权利。
Four novel double-stranded RNA segments were detected in a Verticillium dahliae Kleb. strain (V. dahliae isolate 0-21), a causal fungal agent of Verticillium wilt disease of cotton. Each dsRNA genome segment contains a single large open reading frame (ORF) that encodes a distinctive protein with modest levels of sequence similarities to the corresponding putative proteins in the genus Chrysovirus. These include an RNA-dependent RNA polymerase (RdRp), a coat protein, an undefined replication-related protein and an ovarian tumor domain peptidase. Phylogenetic analysis of the four putative proteins unanimously indicated that they are evolutionarily related to viruses in Chrysovirus. The 5'- and 3'-untranslated regions of the four dsRNAs share highly similar internal sequence and contain conserved sequence stretches of UGAUAAAAAA(/U)UG(/U)AAAAA- (in the 5'-UTR) and -UUUACUACU (in the 3'-UTR), indicating that they have a common virus origin. Indeed, isometric virus-like particles (VLPs) with a diameter of approximately 34 nm were extracted from the fungal mycelia, and the four dsRNA segments were also detected in the virus-like particle (VLP) fraction. These results suggest that the mycovirus with four different dsRNA genome segments from the fungal isolate 0-21 is a new member of the genus Chrysovirus. We named the virus Verticillium dahliae chrysovirus 1 (VdCV1). (C) 2011 Elsevier B.V. All rights reserved.