Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans.
Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans.
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朗格汉斯岛葡萄糖激酶的色谱分离度和动力学表征。
DOI：
10.1073/pnas.80.1.85
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发表时间：
1983
影响因子：
11.1
通讯作者：
Matschinsky,FM
中科院分区：
文献类型：
--
作者：
Meglasson,MD;Burch,PT;Berner,DK;Najafi,H;Vogin,AP;Matschinsky,FM
采用DEAE-Cibacron蓝F3GA琼脂糖层析纯化了朗格汉斯大鼠胰岛葡萄糖激酶（ATP: d -葡萄糖6-磷酸转移酶，EC 2.7.1.2）。酶被洗脱成两个不同的峰。两种酶组分均与葡萄糖呈s型速率依赖关系（希尔系数= 1.5）。葡萄糖的Km值主要部分为5.7 mM，次要部分为4.5 mM。两者都没有磷酸化GlcNAc。通过Sepharose-N-（6-氨基己醇）-GlcNAc亲和层析制备了GlcNAc激酶（ATP:2-乙酰氨基-2-脱氧-d -葡萄糖6-磷酸转移酶，EC 2.7.1.59）富集组分，其对GlcNAc的Km为25微米。胰岛组织中也有多峰己糖激酶（ATP: d -己糖6-磷酸转移酶，EC 2.7.1.1）洗脱。结果与葡萄糖激酶作为胰腺β细胞的葡萄糖传感器的概念一致。
Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for both enzyme fractions. Km values for glucose were 5.7 mM for the major fraction and 4.5 mM for the minor fraction. Neither fraction phosphorylated GlcNAc. A GlcNAc kinase (ATP:2-acetamido-2-deoxy-D-glucose 6-phosphotransferase, EC 2.7.1.59)-enriched fraction, prepared by affinity chromatography on Sepharose-N-(6-aminohexanoyl)-GlcNAc, had a Km of 25 microM for GlcNAc. Islet tissue also contained hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) eluting in multiple peaks. The results are consistent with the concept that glucokinase serves as the glucose sensor of pancreatic beta cells.
由于 N-乙酰基-D-葡萄糖胺激酶，非肝组织中出现明显的“葡萄糖激酶”活性。
DOI：
--
发表时间：
1980
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
M. B. Allen;J. L. Brockelbank;D. G. Walker
通讯作者：
D. G. Walker