Gene expression profiles of oral leukoplakia and carcinoma: genome-wide comparison analysis using oligonucleotide microarray technology.
Gene expression profiles of oral leukoplakia and carcinoma: genome-wide comparison analysis using oligonucleotide microarray technology.
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DOI：
10.3892/ijo.28.3.619
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发表时间：
2006-03
影响因子：
5.2
通讯作者：
Takeshi Odani;D. Ito;Ming-Heng Li;Ai Kawamata;T. Isobe;M. Iwase;M. Nagumo
中科院分区：
文献类型：
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作者：
Takeshi Odani;D. Ito;Ming-Heng Li;Ai Kawamata;T. Isobe;M. Iwase;M. Nagumo
虽然白斑是口腔最常见的癌前病变，但其分子生物学特性很大程度上是未知的。本研究的目的是利用寡核苷酸芯片技术鉴定与其发病和恶变相关的基因。应用Affymetrix基因芯片系统分析了8,800个基因在口腔白斑(n=4)和口腔鳞癌(n=2)中的表达谱，并用RT-PCR对结果进行了验证。在用基因芯片分析的所有白斑中，有8个基因上调(~gt；2.0倍)，10个基因下调(~lt；0.5倍)。特别是，在正常组织和白斑之间，氯化蛋白和角蛋白显示出更大的差异。与白斑相比，18个选择性表达基因中的一些在鳞状细胞癌中表达明显下调。我们的数据提示，细胞骨架网络成分中的基因异常可能与口腔白斑的发生发展有关。
Although leukoplakia is the most common precancerous lesion of the oral cavity, its molecular biological properties are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis and malignant transformation using oligonucleotide microarray technology. The expression profiles of 8,800 genes in human oral leukoplakia (n=4) and oral squamous cell carcinoma (OSCC) (n=2) were analyzed using the Affymetrix GeneChip system and the results were confirmed with RT-PCR. Eight genes were up-regulated (>2.0-fold) and ten were down-regulated (<0.5-fold) in all leukoplakias analyzed with the GeneChip. In particular, loricrin and keratins displayed greater differences between normal tissue and leukoplakia. Some of the 18 alternatively expressed genes were markedly down-regulated in squamous cell carcinoma compared with leukoplakia. Our data suggested that gene abnormalities in cytoskeleton network components might be responsible for the development and progression of oral leukoplakia.