Transcription activator interactions with multiple SWI/SNF subunits
Transcription activator interactions with multiple SWI/SNF subunits
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DOI：
10.1128/mcb.22.6.1615-1625.2002
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发表时间：
2002-03-01
影响因子：
5.3
通讯作者：
Workman, JL
中科院分区：
文献类型：
--
作者：
Neely, KE;Hassan, AH;Workman, JL
我们以前已经表明，酵母SWI/SNF复合物刺激体外转录从染色质模板在ATP依赖性的方式。在这方面，SWI/SNF的功能需要激活剂的存在，它可以直接相互作用，连接激活剂募集SWI/SNF的转录刺激。在这项研究中，我们确定了SWI/SNF亚基介导其与激活剂的相互作用。使用光交联标签转移策略，我们表明，Snf 5，Swi 1，和Swi 2/Snf 2亚基接触的酵母酸性活化剂，GCN 4和HAP 4，在完整的本地SWI/SNF复合物的背景下。此外，我们发现，相同的三个亚基可以单独与酸性激活结构域相互作用，表明每个亚基有助于结合激活剂。此外，降低这些激活剂的激活潜力的突变也减少了其与这些SWI/SNF亚基中的每一个的相互作用。因此，SWI/SNF复合物的三个不同亚基有助于其与活化结构域的相互作用。
We have previously shown that the yeast SWI/SNF complex stimulates in vitro transcription from chromatin templates in an ATP-dependent manner. SWI/SNF function in this regard requires the presence of an activator with which it can interact directly, linking activator recruitment of SWI/SNF to transcriptional stimulation. In this study, we determine the SWI/SNF subunits that mediate its interaction with activators. Using a photo-cross-linking label transfer strategy, we show that the Snf5, Swi1, and Swi2/Snf2 subunits are contacted by the yeast acidic activators, Gcn4 and Hap4, in the context of the intact native SWI/SNF complex. In addition, we show that the same three subunits can interact individually with acidic activation domains, indicating that each subunit contributes to binding activators. Furthermore, mutations that reduce the activation potential of these activators also diminish its interaction with each of these SWI/SNF subunits. Thus, three distinct subunits of the SWI/SNF complex contribute to its interactions with activation domains.