NS-1 GENE-SEQUENCES FROM 8 DENGUE-2 VIRUSES AND THEIR EVOLUTIONARY RELATIONSHIPS WITH OTHER DENGUE-2 VIRUSES
NS-1 GENE-SEQUENCES FROM 8 DENGUE-2 VIRUSES AND THEIR EVOLUTIONARY RELATIONSHIPS WITH OTHER DENGUE-2 VIRUSES
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DOI：
10.1007/bf01314031
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
VITARANA, UT
中科院分区：
文献类型：
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作者：
BLOK, J;GIBBS, AJ;VITARANA, UT
通过使用合成的寡核苷酸引物对病毒RNA进行测序来确定来自5种泰国和3种斯里兰卡登革-2病毒的NS 1基因的核苷酸序列。 结果表明，是相似的四个已发表的登革-2 NS 1序列和这些基因的分类进行了比较，获得的包膜基因的相同病毒。 分类是相似的，表明泰国分离株可以分为两个独立的组，斯里兰卡分离株是不同的。 我们发现，疾病的严重程度，血清学反应（1度或2度），或隔离年和NS 1蛋白序列变异的各个方面之间没有相关性，没有特定的氨基酸变化与毒力相关。 将这些序列与其他地方发表和分类的序列相结合，以提供登革2型病毒分离株的全面E/NS 1基因分类。
The nucleotide sequences of the NS 1 genes from five Thai and three Sri Lankan dengue-2 viruses were determined by sequencing the viral RNA using synthetic oligonucleotide primers. The results were shown to be similar to four published dengue-2 NS 1 sequences and the classification of these genes was compared with the one obtained for the envelope genes of the same viruses. The classification was similar and showed that the Thai isolates could be divided into two separate groups and that the Sri Lankan isolates were distinct. We found no correlation between disease severity, serological response (1-degrees or 2-degrees), or year of isolation and various aspects of NS 1 protein sequence variation; and no particular amino acid changes were correlated with virulence. The sequences were combined with those published and classified elsewhere to provide a comprehensive E/NS 1 gene taxonomy of dengue-2 virus isolates.