Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
复制标题
大脑中表达的基因定义了三种不同的神经元烟碱乙酰胆碱受体。
作者：
P. Nef;Carole Oneyser;C. Alliod;S. Couturier;M. Ballivet
四个基因编码相关的蛋白质亚基，这些亚基在脊椎动物的运动终板上组装形成烟碱乙酰胆碱受体（nAChR）。我们从鸡基因组中分离出了同一基因家族的另外四个成员，其蛋白质产物，称为 α2、α3、α4 和 nα（非α）可能定义了三种不同的神经元 nAChR 亚型。神经元 nAChR 基因具有相同的结构，由六个蛋白质编码外显子组成，并指定与鸡终板 α 亚基最匹配的蛋白质，我们也对其基因进行了表征。编码α4和nα的mRNA转录本在胚胎和成年鸟类大脑中丰富，而α2和α3转录本则稀缺得多。同一组神经元基因可能存在于所有脊椎动物中，因为它们的对应物也在大鼠基因组中被发现。
Four genes encode the related protein subunits that assemble to form the nicotinic acetylcholine receptor (nAChR) at the motor endplate of vertebrates. We have isolated from the chicken genome four additional members of the same gene family whose protein products, termed alpha 2, alpha 3, alpha 4 and n alpha (non‐alpha) probably define three distinct neuronal nAChR subtypes. The neuronal nAChR genes have identical structures consisting of six protein‐coding exons and specify proteins that are best aligned with the chicken endplate alpha subunit, whose gene we have also characterized. mRNA transcripts encoding alpha 4 and n alpha are abundant in embryonic and in adult avian brain, whereas alpha 2 and alpha 3 transcripts are much scarcer. The same set of neuronal genes probably exists in all vertebrates since their counterparts have also been identified in the rat genome.