Upstream CREs participate in the basal activity of minute virus of mice promoter P4 and in its stimulation in ras-transformed cells
Upstream CREs participate in the basal activity of minute virus of mice promoter P4 and in its stimulation in ras-transformed cells
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上游 CRE 参与小鼠启动子 P4 微小病毒的基础活性及其对 ras 转化细胞的刺激
DOI：
10.1128/jvi.69.9.5506-5515.1995
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发表时间：
1995
影响因子：
5.4
通讯作者：
J. Rommelaere
中科院分区：
文献类型：
--
作者：
M. Perros;L. Deleu;J. Vanacker;Z. Kherrouche;N. Spruyt;S. Faisst;J. Rommelaere
The activity of the P4 promoter of the parvovirus minute virus of mice (prototype strain MVMp) is stimulated in ras-transformed FREJ4 cells compared with the parental FR3T3 line. This activation may participate in the oncolytic effect of parvoviruses, given that P4 drives a transcriptional unit encoding cytotoxic nonstructural proteins. Our results suggest that the higher transcriptional activity of promoter P4 in FREJ4 cells is mediated at least in part by upstream CRE elements. Accordingly, mutations in the CRE motifs impair P4 function more strongly in the FREJ4 derivative than in its FR3T3 parent. Further evidence that these elements contribute to hyperactivity of the P4 promoter in the ras transformant is the fact that they form distinct complexes with proteins from FREJ4 and FR3T3 cell extracts. This difference can be abolished by treating the FREJ4 cell extracts with cyclic AMP-dependent protein kinase (PKA) or treating original cultures with a PKA activator. These findings can be linked with two previously reported features of ras-transformed cells: the activation of a PKA-inhibited protein kinase cascade and the reduction of PKA-induced protein phosphorylation. In keeping with these facts, P4-directed gene expression can be up- or downmodulated in vivo by exposing cells to known inhibitors or activators of PKA, respectively.
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ras 对保守序列元件的转录激活需要不同于 c-fos 或 c-jun 的核因子。
DOI：
10.1073/pnas.87.10.3866
发表时间：
1990
影响因子：
11.1
作者：
Owen,RD;Ostrowski,MC
通讯作者：
Ostrowski,MC
细小病毒非结构蛋白表达的限制可能决定EJ-ras转化的大鼠细胞对小鼠微小病毒的敏感性程度。
影响因子：
3.7
作者：
VanHille,B;Duponchel,N;Salomé,N;Spruyt,N;Cotmore,SF;Tattersall,P;Cornelis,JJ;Rommelaere,J
通讯作者：
Rommelaere,J
响应 ras 和 fms 信号通路的增强子元件由两个不同的核因子结合位点组成。
DOI：
10.1210/mend.6.7.1324418
发表时间：
1992
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Reddy,MA;Langer,SJ;Colman,MS;Ostrowski,MC
通讯作者：
Ostrowski,MC
v-ras 和蛋白激酶 C 通过下调核 cAMP 依赖性蛋白激酶 A 使甲状腺细胞去分化。
影响因子：
10.5
作者：
Gallo,A;Benusiglio,E;Bonapace,IM;Feliciello,A;Cassano,S;Garbi,C;Musti,AM;Gottesman,ME;Avvedimento,EV
通讯作者：
Avvedimento,EV
影响因子：
8
作者：
Kondo, T;Minamino, N;Tanaka, N
通讯作者：
Tanaka, N