Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish
Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish
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DOI：
10.1016/j.syapm.2010.02.002
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Jose Figueras, Maria
中科院分区：
文献类型：
--
作者：
Beaz-Hidalgo, Roxana;Alperi, Anabel;Jose Figueras, Maria
对主要从病鱼中分离的119株气单胞菌进行了表型鉴定，并对鉴定结果进行了一致性分析。根据GCAT属特异性基因的分子鉴定结果，只有90株（75.6%）属于气单胞菌属。16 S rDNA-RFLP法能正确鉴定出除少数A. bestiarum、美洲虎A. salmonicida或A.鱼形虫用rpoD基因序列对这3个种的分离进行了正确的评估，结果显示5个菌株具有A. salmonicida属于A. piscicola，有1株为A.野兽正确的表型鉴定仅发生在32（35.5%）的90株。64例中只有14例（21.8%）表型鉴定为A.该属的菌株属于该种。而88%（15/17）的遗传鉴定结果与A.杀鲑菌株对90株气单胞菌进行了表型鉴定，发现A. sobria的一些先前公布的物种特异性性状。经遗传学鉴定，优势种为A. sobria、黑腹拟步行虫A. salmonicida、A. bestiarum、美洲虎A. Alzheimer，A. piscicola和A.媒体，但我们也可以确定最近描述的物种A的新分离物。复盖层这项工作强调了需要依靠16 S rDNA-RFLP方法和管家基因（如rpoD）的测序来正确鉴定气单胞菌菌株。(C)2010年Elsevier GmbH。All rights reserved.
Phenotypicaly identified Aeromonas strains (n=119) recovered mainly from diseased fish were genetically re-identified and the concordance between the results was analysed. Molecular characterization based on the GCAT genus specific gene showed that only 90 (75.6%) strains belonged to the genus Aeromonas. The 16S rDNA-RFLP method identified correctly most of the strains with the exception of a few that belonged to A. bestiarum, A. salmonicida or A. piscicola. Separation of these 3 species was correctly assessed with the rpoD gene sequences, which revealed that 5 strains with the RFLP pattern of A. salmonicida belonged to A. piscicola, as did 1 strain with the pattern of A. bestiarum. Correct phenotypic identification occurred in only 32 (35.5%) of the 90 strains. Only 14 (21.8%) of the 64 phenotypically identified A. hydrophila strains belonged to this species. However, coincident results were obtained in 88% (15/17) of the genetically identified A. salmonicida strains. Phenotypic tests were re-evaluated on the 90 genetically characterized Aeromonas strains and there were contradictions in the species A. sobria for a number of previously published species-specific traits. After genetic identification, the prevailing species were A. sobria, A. salmonicida, A. bestiarum, A. hydrophila, A. piscicola and A. media but we could also identify a new isolate of the recently described species A. tecta. This work emphasizes the need to rely on the 16S rDNA-RFLP method and sequencing of housekeeping genes such as rpoD for the correct identification of Aeromonas strains. (C) 2010 Elsevier GmbH. All rights reserved.