SYMPATHOADRENAL REGULATION OF ADRENOCORTICAL STEROIDOGENESIS
SYMPATHOADRENAL REGULATION OF ADRENOCORTICAL STEROIDOGENESIS
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DOI：
10.3109/07435809509030417
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发表时间：
1995-01-01
影响因子：
2.1
通讯作者：
SCHERBAUM, WA
中科院分区：
文献类型：
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作者：
EHRHARTBORNSTEIN, M;BORNSTEIN, SR;SCHERBAUM, WA
在生理、细胞、分子和形态学水平上研究了交感肾上腺对肾上腺皮质类固醇生成的调节。在保留神经供应的离体灌注猪肾上腺中，比较了神经激活和肾上腺素 (EPI) 对肾上腺皮质类固醇释放的影响。内脏神经激活以及 EPI 灌注引起皮质醇、醛固酮和雄烯二酮的大量释放。在使用或不使用普萘洛尔或酚妥拉明的 EPI 刺激后，研究了培养的牛肾上腺皮质细胞中 P450(scc)、P450(17 α)、P450(c21) 和 P450(11 β) mRNA 的类固醇分泌和积累。与 EPI 一起孵育可刺激类固醇生成并增加所有四种 P450-mRNA 的水平。 β-肾上腺素能拮抗剂普萘洛尔完全阻断EPI的作用，而α-拮抗剂酚妥拉明则没有作用。使用免疫组织化学对肾上腺进行形态学研究。在电子显微镜水平上研究了两种细胞类型的接触区域。皮质和髓质细胞与皮质和嗜铬细胞直接并置紧密交织，为旁分泌相互作用提供了可能性。结论是可以通过交感肾上腺系统刺激皮质类固醇的释放。 EPI 对肾上腺类固醇形成和所有四种 P450-mRNA 积累的刺激作用需要 β-肾上腺素能受体。考虑到皮质和髓质组织的紧密共定位，这种刺激可能是由嗜铬细胞以旁分泌方式介导的。
Sympathoadrenal regulation of adrenocortical steroidogenesis was studied on a physiological, cellular, molecular and morphological level. The effects of nerve activation and of epinephrine (EPI) on adrenal corticosteroid release were compared in isolated perfused pig adrenals with preserved nerve supply. Splanchnic nerve activation as well as perfusion with EPI provoked a siginificant release of cortisol, aldosterone and androstenedione. In cultured bovine adrenocortical cells steroid secretion and accumulation of P450(scc), P450(17 alpha), P450(c21) and P450(11 beta) mRNAs were studied after stimulation with EPI with or without propranolol or phentolamine. Incubation with EPI stimulated steroidogenesis and increased the levels of all four P450-mRNAs. The beta-adrenergic antagonist propranolol totally blocked the effects of EPI while the alpha-antagonist phentolamine had no effect. Using immunohistochemistry, adrenals were studied morphologically. The contact zones of the two cell types were investigated on an electron microscopical level. Cortical and medullary cells were closely interwoven with cortical and chromaffin cells in direct apposition, providing the possibility for paracrine interactions. It is concluded that the release of corticosteroids can be stimulated through the sympatho-adrenal system. The stimulatory action of EPI upon adrenal steroid formation and accumulation of all four P450-mRNAs requires beta-adrenergic receptors. Taking into consideration the close colocalization of cortical and medullary tissue, this stimulation may be mediated by chromaffin cells in a paracrine manner.