Anti-tumour effects of nobiletin, a citrus flavonoid, on gastric cancer include: antiproliferative effects, induction of apoptosis and cell cycle deregulation
Anti-tumour effects of nobiletin, a citrus flavonoid, on gastric cancer include: antiproliferative effects, induction of apoptosis and cell cycle deregulation
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DOI：
10.1111/j.1365-2036.2004.02082.x
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发表时间：
2004-07-01
影响因子：
7.6
通讯作者：
Kitajima, M
中科院分区：
文献类型：
--
作者：
Yoshimizu, N;Otani, Y;Kitajima, M
目的：研究陈皮总黄酮(5，6，7，8，3‘，4’-六甲氧基黄酮)对人胃癌细胞株TMK-1、MKN-45、MKN-74和KATO-III的抗肿瘤作用。材料与方法：四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法、TdT介导的缺口末端标记法和细胞周期分析表明，去甲内酯可通过直接细胞毒、诱导细胞凋亡和调节细胞周期等途径发挥作用。此外，还观察了诺必利汀与常规抗癌药物顺铂联合治疗的疗效。结论：尽管甲基莲心素的有效剂量和给药途径有待进一步研究，但我们的研究表明该化合物可能成功地用于胃癌的治疗。
Aim: To demonstrate the antitumour effects of nobiletin (5,6,7,8,3',4'-hexamethoxyflavone), a citrus flavonoid extracted from Citrus depressa Hayata, on human gastric cancer cell lines TMK-1, MKN-45, MKN-74 and KATO-III.Materials and methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the TdT-mediated dUTP biotin nick-end labelling (TUNEL) method and cell-cycle analysis revealed that nobiletin acted on these cells in several ways, namely by direct cytotoxicity, induction of apoptosis and modulation of cell cycle. The efficacy of combined treatment of nobiletin with a conventional anticancer drug, CDDP, was also examined. Treatment with nobiletin 24 h prior to CDDP administration showed a synergistic effect compared to the control.Conclusions: Although the effective dose and administration route of nobiletin require further investigation, our study represents a potential successful linking of this compound with the treatment of gastric cancer.