Enhancement of Polygalacturonase Production from Enterobacter Aerogenes NBO2 by Submerged Fermentation
Enhancement of Polygalacturonase Production from Enterobacter Aerogenes NBO2 by Submerged Fermentation
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通过深层发酵提高产气肠杆菌 NBO2 多聚半乳糖醛酸酶的产量
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
S. H. Lim
中科院分区：
文献类型：
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作者：
I. Darah;M. Nisha;S. H. Lim
为了提高果胶酶的产量，对产气肠杆菌NBO 2进行了液体深层发酵培养，并对其理化特性进行了研究。优化后的发酵条件为：pH6.5，培养温度37 ℃，接种量3%（v/v）7.4 × 108 cell/ml，搅拌速度250 rpm，果胶浓度1.50%（w/v），酵母膏0.26%作为碳源和氮源，在24 h时，果胶酶产量最高，为18.54 U/mL，菌体生长量为0.43 g/L。发现该细菌分离物的酶产生不依赖于生长。经过理化参数的改善后，产酶量和菌体生长量均有所增加。
The bacterium, Enterobacter aerogenes NBO2 was cultivated in 250 ml Erlenmeyer flask under submerged fermentation and physiochemical characteristics were studied in order to improve the pectinase production. Fermentation condition improved were pH 6.5, cultivation temperature of 37°C, inoculums size of 3% (v/v) 7.4x10 8 cell/ml and agitation speed of 250 rpm, together with pectin concentration of 1.50% (w/v) and 0.26% of yeast extract as carbon and nitrogen sources, respectively had produced the highest pectinase production of about 18.54 U/mL and 0.43 g/L cell growth at 24 hours incubation time. The enzyme production by this bacterial isolate was found not growth dependent. There were increment in enzyme production and cell growth after improvement of physical and chemical parameters.