Fiber-optic bio-sniffer (biochemical gas sensor) for high-selective monitoring of ethanol vapor using 335 nm UV-LED
Fiber-optic bio-sniffer (biochemical gas sensor) for high-selective monitoring of ethanol vapor using 335 nm UV-LED
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DOI：
10.1016/j.snb.2010.03.066
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发表时间：
2010-06-03
影响因子：
8.4
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
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作者：
Kudo, Hiroyuki;Sawai, Masayuki;Mitsubayashi, Kohji
制作并测试了一种高灵敏度、高选择性的连续监测气态酒精的光纤生物嗅探器（生化气体传感器）。生物嗅探器使用乙醇脱氢酶 (ADH) 来识别乙醇。通常，酶在气相中失去活性。生物嗅探器通过应用流通池通过循环磷酸盐缓冲液 (PB) 刷新和润湿酶固定膜，解决了这个问题。生物嗅探器通过测量烟酰胺腺嘌呤二核苷酸 (NADH) 的荧光来测量乙醇蒸汽，烟酰胺腺嘌呤二核苷酸 (NADH) 是由流动池中的酶促反应产生的。为了构建适合现场应用的简化系统，采用高强度紫外发光二极管（UV-LED）作为激发源。使用标准气体发生器对生物嗅探器进行评估。首先，通过研究荧光光谱和荧光特性对PB流速的依赖性来优化生物嗅探器的操作条件。结果，生物嗅探器被证实可用于连续气体监测，校准范围为 0.30-300 ppm。还证实了由于酶的比活性而产生的高选择性。因此，ADH 生物嗅探器预计可用于呼吸分析或医学筛查应用。 (C) 2010 Elsevier B.V. 保留所有权利。
A fiber-optic bio-sniffer (biochemical gas sensor) for continuous monitoring of gaseous alcohol with high sensitivity and high-selectivity was fabricated and tested. The bio-sniffer uses alcohol dehydrogenase (ADH) for recognition of ethanol. Usually, enzyme loses activity in the gas phase. The bio-sniffer solved this problem by applying a flow-cell to refresh and wet the enzyme immobilized membrane by circulating phosphate buffer (PB). The bio-sniffer measures ethanol vapor by measuring Fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by enzymatic reaction at the Flow-cell. In order to construct a simplified system suitable for on-site applications, a high-intensity ultraviolet light emitting diode (UV-LED) was utilized as an excitation source. Assessment of the bio-sniffer was performed using a standard gas generator. First, the operating condition of the bio-sniffer was optimized by investigating Fluorescent spectrum and the dependence of Fluorescent characteristics on Flow-rate of PB. As a result, the bio-sniffer was confirmed to be useful for continuous gas monitoring and the calibration range was 0.30-300 ppm. High-selectivity due to specific activity of enzyme was also confirmed. Thus, the ADH bio-sniffer was expected to be useful for breath analysis or medical screening applications. (C) 2010 Elsevier B.V. All rights reserved.