Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)
Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)
复制标题
东方果蝇桔小实蝇 (Hendel) 核受体基因家族的分子特征和表达谱
DOI：
10.3390/insects11020126
复制
发表时间：
2020-02-01
期刊：
影响因子：
3
通讯作者：
Wang，Jin-Jun
中科院分区：
文献类型：
--
作者：
Yang，Pei-Jin;Chen，Er-Hu;Wang，Jin-Jun
桔小实蝇（Bactrocera dorsalis）是一种对果蔬产业造成巨大经济损失的害虫，其防治是一项重大挑战。核受体（Nuclear receptor, NRs）是一个依赖配体的转录因子超家族，直接与DNA结合，调控下游靶基因的表达。核糖核酸与多种生理过程密切相关，如代谢、繁殖和发育。通过序列检索和分析，共鉴定出21个背孢白僵菌NR基因，所有基因均含有上述两个特征结合域中的至少一个。根据保守序列和系统发育关系，将21个NR基因划分为7个亚家族。NR0亚家族的所有成员和属于NR2E群的BdHR83都缺乏配体结合结构域。BdDSF和BdHR51（也属于nr2e组）和BdE78（属于NR1E组）都缺乏dna结合域。BdDSF和BdHR83序列不完整，未扩增成功。发育和组织特异性表达谱分析表明，19个NR基因的转录水平在卵、幼虫、蛹和成虫以及幼虫和成虫的雄性和雌性组织中存在很大差异。我们的研究结果将有助于更好地理解NR的进化，并扩大我们对背背芽孢杆菌生理学的认识。
The oriental fruit fly (Bactrocera dorsalis) is a pest that causes large economic losses in the fruit and vegetable industry, so its control is a major challenge. Nuclear receptors (NRs) are a superfamily of ligand-dependent transcription factors that directly combine with DNA to regulate the expression of downstream target genes. NRs are closely associated with multiple physiological processes such as metabolism, reproduction, and development. Through sequence searches and analysis, we identified 21 B. dorsalis NR genes, all of which contained at least one of the two characteristic binding domains. On the basis of the conserved sequences and phylogenetic relationships, we divided the 21 NR genes into seven subfamilies. All members of the NR0 subfamily and BdHR83, which belonged to the NR2E group, lacked ligand-binding domains. The BdDSF and BdHR51, which also belonged to the NR2Egroup, and BdE78 (which belonged to the NR1E group) all lacked DNA-binding domains. The BdDSF and BdHR83 sequences were incomplete, and were not successfully amplified. Development- and tissue-specific expression profiling demonstrated that the transcript levels of the 19 NR genes varied considerably among eggs, larva, pupae, and adults, as well as among larval and adult male and female tissues. Our results will contribute to a better understanding of NR evolution and expand our knowledge of B. dorsalis physiology.