Experimental study on the effect of flow in microfluidic channel on bovine sperm navigation
Experimental study on the effect of flow in microfluidic channel on bovine sperm navigation
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微流通道内流动对牛精子导航影响的实验研究
DOI：
10.1016/j.jbiomech.2021.110290
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发表时间：
2021
影响因子：
2.4
通讯作者：
Renta Murakami and Yasutaka Hayamizu
中科院分区：
文献类型：
--
作者：
Toru Hyakutake;Kenta Sugita;Shota Ujifuku;Rintaro Sakurai;Renta Murakami and Yasutaka Hayamizu
由于其复杂的过程，哺乳动物精子在女性生殖道中的导航机制尚不清楚。 This study performed anin vitroexperiment using the microfluidic channel with two reservoirs to investigate the effect of fluid flow on the swimming properties of the bovine sperm.制造的通道的宽度和高度分别为 200 和 20 μm。微通道中的流动是由于两个储层之间的水头差而发生的。具有正流变性的精子在游到下游水库后沿与通道中流动相反的方向行进。这项研究的重点是微流体通道中的流动对精子活力的影响。据观察，精子大多沿着通道壁移动，并聚集在远离下游水库的壁附近。通道内流体流动的存在导致正向趋变性精子比例的增加。 Furthermore, the experimental results indicated that the motility of sperm swimming against the flow along the wall increased away from the downstream reservoir.这些结果将为理解体内受精的精子导航机制提供有用的信息。
The navigation mechanism of mammalian sperm in the female reproductive tract is unclear owing to its complex process. This study performed anin vitroexperiment using the microfluidic channel with two reservoirs to investigate the effect of fluid flow on the swimming properties of the bovine sperm. The width and height of the manufactured channel were 200 and 20 μm, respectively. The flow in the microchannel occurs because of the hydraulic head difference between the two reservoirs. Sperm with positive rheotaxis proceed in the opposite direction of the flow in the channel after swimming up the downstream reservoir. This study focused on the effect of the flow in the microfluidic channel on sperm motility. It was observed that sperm mostly moved along the channel wall and accumulated near the wall away from the downstream reservoir. The existence of fluid flow in the channel brought about an increase in the ratio of the sperm with positive rheotaxis. Furthermore, the experimental results indicated that the motility of sperm swimming against the flow along the wall increased away from the downstream reservoir. These results will provide useful information to understand the mechanism of sperm navigation forin vivofertilization.