Sensitive and Selective Label-Free DNA Detection by Conjugated Polymer-Based Microarrays and Intercalating Dye
Sensitive and Selective Label-Free DNA Detection by Conjugated Polymer-Based Microarrays and Intercalating Dye
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DOI：
10.1021/cm800333r
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发表时间：
2008-04
影响因子：
8.6
通讯作者：
Kangwon Lee;K. Maisel;J. Rouillard;E. Gulari;Jinsang Kim
中科院分区：
文献类型：
--
作者：
Kangwon Lee;K. Maisel;J. Rouillard;E. Gulari;Jinsang Kim
我们已经证明了使用共轭聚合物和嵌入染料SYBR绿色I的无标记和信号放大的DNA微阵列。从共轭聚合物到染料的有效FRET产生大的信号放大，使得在不损失良好选择性的情况下，实现了对亚皮摩尔浓度的靶DNA的灵敏检测。
We have demonstrated label-free and signal-amplifying DNA microarray using a conjugated polymer and an intercalating dye SYBR green I. Efficient FRET from the conjugated polymer to the dye produced large signal amplification so that without losing good selectivity, sensitive detection of subpicomolar concentrations of the target DNA was achieved.