Selective solid-phase extraction of catecholamines from plasma using nanofibers doped with crown ether and their quantitation by HPLC with electrochemical detection
Selective solid-phase extraction of catecholamines from plasma using nanofibers doped with crown ether and their quantitation by HPLC with electrochemical detection
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使用掺杂冠醚的纳米纤维选择性固相萃取血浆中的儿茶酚胺，并通过 HPLC 和电化学检测进行定量
DOI：
10.1007/s00216-016-9596-7
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Zhang, WanQi
中科院分区：
文献类型：
--
作者：
Chen, LiQin;Zhu, XingHua;Zhang, WanQi
以二苯并-18-冠-6为亚基，采用静电纺丝法制备了高分子冠醚和聚苯乙烯（PCE-PS）复合纳米纤维。此外，该复合吸附剂被用作选择性提取血浆中的儿茶酚胺，即多巴胺（DA），去甲肾上腺素（NE）和肾上腺素（E）的吸附剂。在血浆样品的蛋白质沉积后，用缓冲溶液将上清液调节至中性pH。然后，加载混合物并推动其通过填充有复合纳米纤维吸附剂的柱，并用50 μ L乙酸洗脱分析物。血浆样品净化方法的有效性通过高效液相色谱电化学检测器（HPLC-ECD）进行了验证。PCE-PS填充柱可用于临床，因为它提供了一种方便和选择性的方法来评价人血浆中的儿茶酚胺。
A simple and convenient preparation method has been developed for polymeric crown ethers containing the dibenzo-18-crown-6 subunit and electrospun composite nanofibers composed of polymeric crown ethers with polystyrene (PCE-PS). Furthermore, this composite nanofiber was used as a sorbent for selective extraction of plasma catecholamines, viz. dopamine (DA), norepinephrine (NE), and epinephrine (E). After the protein deposition of the plasma sample, the supernatant was adjusted to neutral pH with buffer solution. Then, the mixture was loaded and pushed through a column packed with composite nanofiber sorbent, and the analytes were eluted with 50 mu L of acetic acid. The effectiveness of the plasma sample cleanup method was verified by high-performance liquid chromatography with electrochemical detection (HPLC-ECD). The PCE-PS packed column may prove clinically useful, as it provides a convenient and selective method for evaluation of human plasma catecholamines.