In vivo, three‐dimensional microscopy of trabecular bone
In vivo, three‐dimensional microscopy of trabecular bone
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DOI：
10.1002/jbmr.5650100213
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发表时间：
1995-02
影响因子：
6.2
通讯作者：
J. Kinney;N. Lane;D. Haupt
中科院分区：
文献类型：
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作者：
J. Kinney;N. Lane;D. Haupt
骨质疏松症与低骨量和骨质脆弱性增加有关，在美国每年约有120万例骨折。我们使用三维（3 - D） X射线断层扫描显微镜对六只Sprague-Dawley大鼠胫骨近端骨小梁结构进行了活体成像。然后，其中三只大鼠被切除卵巢以诱导雌激素消耗，其余三只作为对照。五周后，对胫骨进行了重建。切除卵巢的大鼠失去了大约65%的骨小梁体积，而对照组的变化微不足道。欧拉特征测量的骨小梁连通性密度与骨小梁体积呈线性相关，即使在去卵巢大鼠中也是如此。低雌激素性骨质流失表现为连通性大大降低和小梁构件减少，但不表现为小梁变薄。在体内对骨小梁三维结构的连续变化进行显微成像的能力，将有助于探索低雌激素性骨质流失的早期阶段，并更快地测试针对这一重大公共卫生问题的新临床治疗方法的有效性。
Osteoporosis, which is correlated with low bone mass and increased bone fragility, is responsible for about 1.2 million fractures per year in the United States. We have used the three‐dimensional (3‐D), X‐ray tomographic microscope to image the trabecular bone architecture of the proximal tibias of six Sprague—Dawley rats in vivo. Three of these rats were then ovariectomized to induce estrogen depletion, and three remained as controls. Five weeks later, the tibias were reimaged. The ovariectomized rats lost approximately 65% of their trabecular bone volume as compared with an insignificant change in the control rats. The connectivity density of the trabecular bone, as measured by the Euler characteristic, was linearly correlated with trabecular bone volume, even in the ovariectomized rats. Hypoestrogenemic bone loss manifested itself in greatly reduced connectivity and fewer trabecular elements, but not in thinning of trabeculae. The ability to microscopically image sequential changes in the 3‐D architecture of trabecular bone in vivo will allow exploration of the earliest stages of hypoestrogenemic bone loss and to more rapidly test the effectiveness of new clinical treatments for this major public health problem.