A Qualitative Baiting Technique for Selective Isolation and DNA Diagnosis of Rhizoctonia spp,Causal AGents of Rice Sheat Diseeases,from Soil
A Qualitative Baiting Technique for Selective Isolation and DNA Diagnosis of Rhizoctonia spp,Causal AGents of Rice Sheat Diseeases,from Soil
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水稻纹枯病病原丝核菌从土壤中选择性分离和 DNA 诊断的定性诱饵技术
DOI：
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发表时间：
2003
影响因子：
0.6
通讯作者：
松原 賢
中科院分区：
文献类型：
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作者：
M. Matsumoto;松原 賢
建立了水稻纹枯病病原菌选择性分离和DNA诊断的诱饵技术。用11个植物茎段和含有苯菌灵和甲霜灵的选择性培养基，从人工接种和自然侵染的土壤中分离。用50 μ g/ml甲霜灵浸泡水稻、芦竹和小麦茎段，成功地分离出水稻锈病菌（R. solani AG 2，R.稻R.或~/zae-sativae和R.此外，用1007 tg/ml甲霜灵浸泡水稻和小麦茎段，成功地提取了所有丝核菌属的基因组DNA。甲霜灵未处理的茎定殖在显着更高的数字，并包含在显着更高浓度的DNA的这些丝核菌分离物比未处理的茎。未经处理的茎也定殖分离物和提取的DNA来自其他常见的土壤栖息真菌。
A baiting technique was evolved for selective isolation and DNA diagnosis of causal agents of rice sheath diseases by Rhizoctonia spp. from artificially inoculated and naturally infested soils using eleven plant stem segments and selective medium containing benomyl and metalaxyl. Rice, mat rush and wheat stem segments soaked in metalaxyl at 50~tg/ml were successfully used to isolate R, solani AG 1, R. solani AG 2, R. oryzae, R. or~/zae-sativae and R, fumigate from artificially infested soils. Moreover, rice and wheat stem segments soused in metalaxyl at 1007tg/ml were successfully used to extract genomic DNA derived from all Rhizoctonia spp. Metalaxyl untreated stems were colonized in significantly higher numbers and contained in significantly higher concentration of DNA of these Rhizoctonia isolates than untreated stems. Untreated stems were also colonized isolates and extracted DNA derived from other common soil-inhabiting fungi.