PRP16 IS AN RNA-DEPENDENT ATPASE THAT INTERACTS TRANSIENTLY WITH THE SPLICEOSOME
PRP16 IS AN RNA-DEPENDENT ATPASE THAT INTERACTS TRANSIENTLY WITH THE SPLICEOSOME
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DOI：
10.1038/349494a0
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发表时间：
1991-02-07
期刊：
影响因子：
64.8
通讯作者：
GUTHRIE, C
中科院分区：
文献类型：
--
作者：
SCHWER, B;GUTHRIE, C
剪接体的组装是一个依赖ATP的过程。 剪接因子PRP 16包含几个定义eIF-4A样ATP依赖性RNA解旋酶家族的基序的变体。 该蛋白现已被纯化，并显示出RNA依赖性ATP酶活性。 PRP 16是体外剪接反应的第二个催化步骤所必需的。 该功能需要ATP结合和/或水解，这似乎伴随着蛋白质从剪接体的释放。 PRP 16可能是一组剪接因子的原型，这些剪接因子使用ATP来驱动构象变化的循环。
The assembly of the spliceosome is an ATP-dependent process. The splicing factor PRP16 contains variations of several motifs that define the eIF-4A-like ATP-dependent RNA helicase family. The protein has now been purified and shown to exhibit RNA-dependent ATPase activity. PRP16 is required specifically for the second catalytic step of the splicing reaction in vitro. This function requires ATP binding and/or hydrolysis, which appears to be concomitant with release of the protein from the spliceosome. PRP16 may be the prototype for a set of splicing factors which use ATP to drive a cycle of conformational changes.