A novel platform device for rodent echocardiography.
A novel platform device for rodent echocardiography.
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一种用于啮齿动物超声心动图的新型平台装置。
DOI：
10.1093/ilar.49.2.e1
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发表时间：
2007
期刊：
影响因子：
2.5
通讯作者：
R. Robbins
中科院分区：
文献类型：
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作者：
I. Kutschka;Ahmad Y. Sheikh;R. Sista;S. Hendry;H. Chun;G. Hoyt;Werner Kutschka;M. Pelletier;T. Quertermous;Joseph C. Wu;R. Robbins
由于技术的差异，从啮齿动物中获取超声心动图数据存在很大的差异。我们假设专用成像平台可以帮助技术标准化并提高所获得图像的质量。我们构建了一个装置，该装置由吊杆安装的钢平台框架（25 x 35 x 3 cm）组成，其上安装有透明聚乙烯膜。将动物置于膜上，并通过集成端口接受持续吸入麻醉。该膜允许从动物下方定位探头，以在左侧卧位或俯卧位获得标准回波视图。该框架可以设置在任何所需的角度范围从0至360度沿着无论是长轴或短轴。使用该平台对成年雄性Sprague-Dawley大鼠（n = 5）进行超声心动图（General Electric，Vivid 7，14 MHz）。该设备允许动物的最佳定位，以进行各种标准超声心动图测量。所有动物的评价显示，两名不同操作员和时间点之间的变异性极小。我们测试了该设备的可行性，以支持评估心脏功能的疾病模型，通过评估一个单独的队列的成年雄性自发性高血压大鼠（n = 5），接受左前降支冠状动脉结扎。连续超声心动图显示术后240天缩短分数和射血分数显著降低（p < 0.01）。我们的新型成像平台允许从大鼠中持续收集高质量的超声心动图数据。未来的研究将集中在改进这项技术，以允许在小动物疾病模型中进行标准化的高通量超声心动图分析。
Acquisition of echocardiographic data from rodents is subject to wide variability due to variations in technique. We hypothesize that a dedicated imaging platform can aid in standardization of technique and improve the quality of images obtained. We constructed a device consisting of a boom-mounted steel platform frame (25 x 35 x 3 cm) on which a transparent polyethylene membrane is mounted. The animal is placed onto the membrane and receives continual inhaled anesthesia via an integrated port. The membrane allows for probe positioning from beneath the animal to obtain standard echo-views in left lateral decubitus or prone positions. The frame can be set at any desired angle ranging from 0 to 360 degrees along either the long or short axis. Adult male Sprague-Dawley rats (n = 5) underwent echocardiography (General Electric, Vivid 7, 14 MHz) using the platform. The device allowed for optimal positioning of animals for a variety of standard echocardiographic measurements. Evaluations among all animals showed minimal variability between two different operators and time points. We tested the feasibility of the device for supporting the assessment of cardiac function in a disease model by evaluating a separate cohort of adult male spontaneously hypertensive rats (n = 5) that underwent left anterior descending coronary artery ligation. Serial echocardiography demonstrated statistically significant decreases of fractional shortening and ejection fraction (p < 0.01) 240 days after surgery. Our novel imaging platform allowed for consistent collection of high-quality echocardiographic data from rats. Future studies will focus on improving this technology to allow for standardized high-throughput echocardiographic analysis in small animal models of disease.