Rate process analysis of thermal damage in cartilage
Rate process analysis of thermal damage in cartilage
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DOI：
10.1088/0031-9155/48/1/302
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发表时间：
2003-01-07
影响因子：
3.5
通讯作者：
Wong, BJF
中科院分区：
文献类型：
--
作者：
Díaz, SH;Nelson, JS;Wong, BJF
腕关节激光热成型（CLT）是一种新的外科手术，允许在。原位治疗头颈部畸形，发病率低于传统方法。虽然一些动物和人类研究已经显示出有希望的结果，但CLT的临床可行性取决于软骨细胞活力的保存，这尚未得到广泛研究。本文描述了热对兔鼻软骨细胞的损伤。损伤被建模为一阶速率过程，其中两个实验导出的系数A = 1.2 × 10（70）s（-1）和E-a = 4.5 × 10（5）J mole（-1）通过量化组织样品中健康软骨细胞浓度的降低作为暴露于恒温水浴的时间的函数来确定。浸泡后，软骨细胞从基质中酶促分离，并用双组分荧光染料染色。染料结合细胞核DNA的差异取决于软骨细胞的活力。使用流式细胞仪检测差异细胞荧光以确定每个样品中活细胞和死细胞的百分比。因此，损伤动力学模型，可用于预测的发病，范围和严重程度的细胞损伤热暴露。
Cartilage laser thermoforming (CLT) is a new surgical procedure that allows in. situ treatment of deformities in the head and neck with less morbidity than traditional approaches. While some animal and human studies have shown promising results, the clinical feasibility of CLT depends on preservation of chondrocyte viability, which has not been extensively studied. The present paper characterizes cellular damage due to heat in rabbit nasal cartilage. Damage was modelled as a first order rate process for which two experimentally derived coefficients, A = 1.2 x 10(70) s(-1) and E-a = 4.5 x 10(5) J mole(-1), were determined by quantifying the decrease in concentration of healthy chondrocytes in tissue samples as a function of exposure time to constant-temperature water baths. After immersion, chondrocytes were enzymatically isolated from the matrix and stained with a two-component fluorescent dye. The dye binds nuclear DNA differentially depending upon chondrocyte viability. A flow cytometer was used to detect differential cell fluorescence to determine the percentage of live and dead cells in each sample. As a result, a damage kinetic model was obtained that can be used to predict the onset, extent and severity of cellular injury to thermal exposure.