Factors affecting cryopreservation-associated damages in sperm motility of cockerels (Gallus gallus domesticus)
Factors affecting cryopreservation-associated damages in sperm motility of cockerels (Gallus gallus domesticus)
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DOI：
10.1080/00071668.2022.2124099
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发表时间：
2023-01-23
影响因子：
2
通讯作者：
Asano，A.
中科院分区：
文献类型：
--
作者：
Sushadi，P. S.;Kuwabara，M.;Asano，A.
1. 在低温保存期间，精子暴露在严重的渗透压力下，导致家禽的受精能力受损，包括运动性和生存能力。精子的渗透耐受性受多种细胞外因素的调节，在鸟类中差异很大，导致渗透损伤的性质不确定。尾巴弯曲是过度渗透胁迫导致细胞肿胀的主要反应。然而，导致尾巴弯曲的潜在机制在很大程度上是未知的。本研究探讨了渗透胁迫与解冻后运动之间的关系，特别关注Na+/K+ atp酶（NKA）在尾巴弯曲反应中的作用。冷冻保存的精子在37°C时表现出活力迅速下降。温度变化和渗透应力的共同作用是导致尾翼弯曲的主要因素。这项工作验证了一个已知与其他物种解冻后尾巴异常有关的假设，并发现可能会发生不伴随凋亡反应的冷休克。3 .瓦巴因抑制Na+/K+ atp酶活性可减轻新鲜精子和解冻后精子的弯尾反应。这些结果表明，温度变化和渗透胁迫的结合对鸡精子解冻后运动能力的降低有主要影响，特别是对鸡精子尾巴弯曲反应中NKA活性的影响。这些结果为建立冷冻保存方法以确保鸡精子的最佳受精潜力提供了基础。
1. Sperm are exposed to severe osmotic stress during cryopreservation, which results in impairment of fertilisation ability, including motility and viability, in poultry. Sperm osmotolerance is regulated by many extracellular factors and varies widely in birds, leading to uncertainty in the nature of the osmotic injury.2. Tail bending is a primary response resulting from cell swelling from excessive osmotic stress. However, the underlying mechanism responsible for tail bending is largely unknown. This study examined the relationship between osmotic stress and post-thaw motility, with a particular focus on the role of Na+/K+ATPase (NKA) in the tail bending response.3. Cryopreserved sperm exhibited rapidly reduced motility when maintained at 37°C. The combination of temperature change and osmotic stress was a primary factor responsible for tail bending. This work tested a hypothesis known to be associated with post-thaw tail abnormality in other species and found that cold shock, that is not accompanied by an apoptotic response, may occur. Ouabain inhibition of Na+/K+ATPase activity alleviated the tail bending response in fresh and post-thaw sperm.4. These results demonstrated that the combination of temperature change and osmotic stress has a primary impact on the reduction of post-thaw motility, with a particular role in NKA activity, in the tail bending response of chicken sperm. These results provide a foundation for establishing cryopreservation methodology to ensure the optimal fertilisation potential of cryopreserved chicken sperm.