Modulating cellular behaviors through surface nanoroughness
Modulating cellular behaviors through surface nanoroughness
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通过表面纳米粗糙度调节细胞行为
DOI：
10.1039/c2jm32007j
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Zhou, Shaobing
中科院分区：
文献类型：
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作者：
Luo, Chao;Li, Long;Zhou, Shaobing
近年来，纳米尺度表面形貌对细胞功能的影响越来越受到人们的关注。本文采用静电纺丝的方法，通过简单地调整聚合物溶液的浓度来改变纳米纤维膜的表面纳米粗糙度。采用扫描电子显微镜（SEM）和原子力显微镜（AFM）对其表面形貌进行了表征。通过与大鼠骨髓间充质干细胞（rBMSCs）共培养，从细胞粘附、扩散、增殖和分化等方面进一步研究不同表面纳米粗糙度对细胞行为的影响。研究了表面纳米粗糙度对体内皮下骨形成的影响，并用H&E和Masson三色染色染色组织学切片。实验结果表明，微珠的存在不会对细胞的生长产生负面影响，而且相对于光滑的表面，微珠表面的适度纳米粗糙度可以调节和促进细胞的行为。因此，我们认为具有合适纳米粗糙度的电纺纳米纤维作为组织工程支架具有很大的生物医学应用潜力。
The influence of nanoscale surface topographical features on cell functions has attracted more and more attention in the last few years. Herein, the surface nanoroughness on a nanofiber membrane was engineered with an electrospinning approach by simply adjusting the concentration of the polymer solution. The surface topographical features were characterized with scanning electron microscopy (SEM) and atomic force microscopy (AFM). The impact of different surface nanoroughness on cellular behavior was further investigated from cell adhesion, spreading, proliferation and differentiation, through co-culturing with rat bone marrow mesenchymal stem cells (rBMSCs). Investigation of the effect of surface nanoroughness on subcutaneous bone formation in vivo was also performed, and a histological section was dyed with H&E and Masson's trichrome staining. The distinctive acquired result shows that the existence of beads on electrospun nanofibers does not bring any negative effect on cell growth, and moreover the moderate surface nanoroughness can modulate and facilitate cellular behaviors compared with the smooth surface. Therefore, we think that the electrospun nanofibers with suitable nanoroughness can have great potential for biomedical applications as a tissue engineering scaffold.