Divergent and convergent modes of interaction between wheat and Puccinia graminis f. sp. tritici isolates revealed by the comparative gene co-expression network and genome analyses.
Divergent and convergent modes of interaction between wheat and Puccinia graminis f. sp. tritici isolates revealed by the comparative gene co-expression network and genome analyses.
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DOI：
10.1186/s12864-017-3678-6
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发表时间：
2017-04-12
期刊：
影响因子：
4.4
通讯作者：
Akhunov E
中科院分区：
文献类型：
--
作者：
Rutter WB;Salcedo A;Akhunova A;He F;Wang S;Liang H;Bowden RL;Akhunov E
两个相对的进化约束对植物病原体施加压力：一个使病毒因素多样化以逃避植物防御，另一个是保留病毒因素，以维持与植物宿主保持兼容的相互作用至关重要。促进了与相同的小麦线相互作用，我们对两种北美的graminis f进行了比较的基因组分析（PGT）。
将分泌的候选效应基因中的隔离间差异与植物 - 病原基因共表达网络（GCN）的保护水平（GCN）进行了比较。基因完成和序列差异。两种分离株的途径。相似的小麦感染途径，不同途径的植物病原体相互作用的方式有所不同，某些途径与配置的一组相关效应子和其他与分歧或分离株特异性效应相关的。
我们的数据表明，在物种水平上，病原体可能会维持能够靶向同一植物宿主途径的效应集。作为潜水病毒策略的基础，并创造了某些效应子组突变不会对病原体感染宿主的能力产生重大影响的条件。
本文的在线版本（DOI：10.1186/S12864-017-3678-6）包含补充材料，可供授权用户使用。
Two opposing evolutionary constraints exert pressure on plant pathogens: one to diversify virulence factors in order to evade plant defenses, and the other to retain virulence factors critical for maintaining a compatible interaction with the plant host. To better understand how the diversified arsenals of fungal genes promote interaction with the same compatible wheat line, we performed a comparative genomic analysis of two North American isolates of Puccinia graminis f. sp. tritici (Pgt). The patterns of inter-isolate divergence in the secreted candidate effector genes were compared with the levels of conservation and divergence of plant-pathogen gene co-expression networks (GCN) developed for each isolate. Comprative genomic analyses revealed substantial level of interisolate divergence in effector gene complement and sequence divergence. Gene Ontology (GO) analyses of the conserved and unique parts of the isolate-specific GCNs identified a number of conserved host pathways targeted by both isolates. Interestingly, the degree of inter-isolate sub-network conservation varied widely for the different host pathways and was positively associated with the proportion of conserved effector candidates associated with each sub-network. While different Pgt isolates tended to exploit similar wheat pathways for infection, the mode of plant-pathogen interaction varied for different pathways with some pathways being associated with the conserved set of effectors and others being linked with the diverged or isolate-specific effectors. Our data suggest that at the intra-species level pathogen populations likely maintain divergent sets of effectors capable of targeting the same plant host pathways. This functional redundancy may play an important role in the dynamic of the “arms-race” between host and pathogen serving as the basis for diverse virulence strategies and creating conditions where mutations in certain effector groups will not have a major effect on the pathogen’s ability to infect the host. The online version of this article (doi:10.1186/s12864-017-3678-6) contains supplementary material, which is available to authorized users.