SIGNALING DOWNSTREAM OF ACTIVATED MAMMALIAN NOTCH
SIGNALING DOWNSTREAM OF ACTIVATED MAMMALIAN NOTCH
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DOI：
10.1038/377355a0
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发表时间：
1995-09-28
期刊：
影响因子：
64.8
通讯作者：
ISRAEL, A
中科院分区：
文献类型：
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作者：
JARRIAULT, S;BROU, C;ISRAEL, A
Noch属于一个跨膜蛋白家族，从苍蝇到脊椎动物都广泛保守，被认为参与了细胞命运的决定。在果蝇中，无毛(SU(H))基因(1，2)的抑制子和裂解(E(SPL))复合体的增强子(E(SPL))基因编码基本螺旋-环-螺旋型(3，4)蛋白，参与了Notch信号通路。E(SPL)的哺乳动物同源物，如小鼠毛发增强剂Split(HES-1)(5)，已经被分离出来。HES-1和小鼠Notch的胞内区(MNotch)都能阻断MyoD诱导的肌肉发生(5-7)。在这里，我们展示了mNotch激活形式与人类SU(H)，KBF2/RBP-J kappa的类似物(参考文献8，9)，并通过HES-1启动子的KBF2结合位点发挥转录激活因子的作用。
NOTCH belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene(1,2) and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix-loop-helix type(3,4) have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1)(5), have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis(5-7). Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-J kappa (refs 8,9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.