Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori
Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori
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DOI：
10.1073/pnas.0407596101
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发表时间：
2004-11-23
影响因子：
11.1
通讯作者：
Nishioka, T
中科院分区：
文献类型：
--
作者：
Sakurai, T;Nakagawa, T;Nishioka, T
雌性飞蛾释放的性信息素在同种雄性飞蛾触角的嗅觉感受器中具有高特异性和敏感性。蚕蛾 Bombyx mori 中的 Bombykol 是第一个被鉴定的性信息素。在这里，我们鉴定了一种男性特有的 G 蛋白偶联嗅觉受体基因，家蚕嗅觉受体 1 (BmOR-1)，它似乎编码安必可受体。 BmOR-1基因位于Z性染色体上，具有8个外显子/7个内含子结构，在蛹晚期和成虫阶段在雄蛾触角的信息素受体神经元中表现出雄性特异性表达。 Bombykol 刺激表达 BmOR-1 和 BmGalphaq 的非洲爪蟾卵母细胞，在两电极电压钳记录上引发强烈的剂量依赖性内向电流，证明 Bombykol 与 BmOR-1 的结合导致 BmGalphaq 介导的信号级联的激活。感染 BmOR-1 重组杆状病毒的雌性飞蛾的触角显示出对家蚕的电生理反应，但对家蚕没有表现出电生理反应。这些结果证明 BmOR-1 是一种识别蚕豆素的 G 蛋白偶联性信息素受体。
Sex pheromones released by female moths are detected with high specificity and sensitivity in the olfactory sensilla of antennae of conspecific males. Bombykol in the silkmoth Bombyx mori was the first sex pheromone to be identified. Here we identify a male-specific G protein-coupled olfactory receptor gene, B. mori olfactory receptor 1 (BmOR-1), that appears to encode a bombykol receptor. The BmOR-1 gene is located on the Z sex chromosome, has an eight-exon/seven-intron structure, and exhibits male-specific expression in the pheromone receptor neurons of male moth antenna during late pupal and adult stages. Bombykol stimulation of Xenopus laevis oocytes expressing BmOR-1 and BmGalphaq elicited robust dose-dependent inward currents on two-electrode voltage clamp recordings, demonstrating that the binding of bombykol to BmOR-1 leads to the activation of a BmGalphaq-mediated signaling cascade. Antennae of female moths infected with BmOR-1-recombinant baculovirus showed electrophysiological responses to bombykol but not to bombykal. These results provide evidence that BmOR-1 is a G protein-coupled sex pheromone receptor that recognizes bombykol.