Morphological and Molecular Characterization of Adult Midgut Compartmentalization in Drosophila
Morphological and Molecular Characterization of Adult Midgut Compartmentalization in Drosophila
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DOI：
10.1016/j.celrep.2013.04.001
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发表时间：
2013-05-01
期刊：
影响因子：
8.8
通讯作者：
Lemaitre, Bruno
中科院分区：
文献类型：
--
作者：
Buchon, Nicolas;Osman, Dani;Lemaitre, Bruno
虽然肠道是真后生动物的一个中心器官，对生物体的健康至关重要，但我们对其形态和分子决定因素的理解仍然是初步的。在这里，我们提供了一个全面的地图集果蝇成年中肠。具体来说，我们发现了一个细粒度的区域组织，由14个亚区具有不同的形态，组织学和遗传特性。我们还表明，果蝇肠道区域化的定义后，成年出现，保持稳定的整个生命，并重新建立急性组织损伤。此外，我们表明，这种中肠区室化是通过泛中肠和区域化的转录因子之间的相互作用，与空间活动的形态发生。有趣的是，中肠区室化的破坏导致肠道稳态的丧失，其特征在于干细胞增殖和异常免疫应答的增加。我们对果蝇中肠区室化的综合分析为后生动物肠道区域化的保守机制提供了见解。
Although the gut is a central organ of Eumetazoans and is essential for organismal health, our understanding of its morphological and molecular determinants remains rudimentary. Here, we provide a comprehensive atlas of Drosophila adult midgut. Specifically, we uncover a fine-grained regional organization consisting of 14 subregions with distinct morphological, histological, and genetic properties. We also show that Drosophila intestinal regionalization is defined after adult emergence, remains stable throughout life, and reestablishes following acute tissue damage. Additionally, we show that this midgut compartmentalization is achieved through the interplay between pan-midgut and regionalized transcription factors, in concert with spatial activities of morphogens. Interestingly, disruption of the midgut compartmentalization leads to a loss of intestinal homeostasis characterized by an increase in stem cell proliferation and aberrant immune responses. Our integrative analysis of Drosophila midgut compartmentalization provides insights into the conserved mechanisms underlying intestinal regionalization in metazoans.