^<1>H, ^<15>N and ^<13>C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein.
^<1>H, ^<15>N and ^<13>C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein.
复制标题
粟酒裂殖酵母Sin1蛋白中间结构域保守区的^ 1 H、^ 15 N和^ 13 C共振归属。
DOI：
10.1007/s12104-014-9550-6
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发表时间：
2014
影响因子：
0.9
通讯作者：
Kojima C
中科院分区：
文献类型：
--
作者：
Kataoka S;Furuita K Hattori Y;Kobayashi N;Ikegami T;Shiozaki K;Fujiwara T;Kojima C
sapk相互作用蛋白1 （Sin1）是雷帕霉素（TOR）复合物2 （TORC2）靶点的重要组成部分。TOR是一种丝氨酸/苏氨酸特异性蛋白激酶，形成功能不同的蛋白质复合物，称为TORC1和TORC2。TORC2，从酵母到人类保守，磷酸化agc家族蛋白激酶，并具有许多细胞功能，包括调节肌动蛋白细胞骨架。TORC2的Sin1亚基是TORC2与底物结合所必需的，而Sin1中间区域（CRIM）的保守区域在TORC2的底物识别中很重要。本文报道了裂变酵母schizosaccharomyces pombeSin1（氨基酸247-400）（Sin1CRIM）的1h，13C和15n共振分配，该酵母具有CRIM结构域。这些数据有助于确定sin1crim的结构，并了解sin1crim与TORC2底物的相互作用。
SAPK-interacting protein 1 (Sin1) is an important component of the target of rapamycin (TOR) complex 2 (TORC2). TOR is a serine/threonine-specific protein kinase and forms functionally distinct protein complexes referred to as TORC1 and TORC2. TORC2, conserved from yeast to humans, phosphorylates AGC-family protein kinases and has many cellular functions including the regulation of actin cytoskeleton. The Sin1 subunit of TORC2 is required for the binding of TORC2 to substrates, and the conserved region in the middle (CRIM) domain of Sin1 is important in the substrate recognition of TORC2. Here, we report on the1H,13C and15N resonance assignments of fission yeastSchizosaccharomyces pombeSin1 (amino acids 247–400) (Sin1CRIM), which possesses the CRIM domain. These data contribute toward the structure determination of Sin1CRIMand an understanding of the interactions of Sin1CRIMwith substrates of TORC2.