Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells
Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells
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DOI：
10.1007/978-1-4939-3603-8_18
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Fu, Yang-Xin
中科院分区：
文献类型：
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作者：
Guo, Xiaohuan;Muite, Kevin;Fu, Yang-Xin
最近的研究已经确定了几个相关但不同的先天性淋巴样细胞(ILCs)群体，它们相对丰富地存在于肠道粘膜中，并保护宿主免受各种感染。其中，第3组ILC(ILC3)产生淋巴毒素和IL-22，在免疫系统的发育和宿主与细菌的相互作用中发挥重要作用。在这里，我们描述了从小鼠肠道中分离和纯化ILC3s的方法，以及将纯化的ILC3s过继转移到受体小鼠体内的方法。
Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.