Effects of modified digestion schemes on the identification of proteins from complex mixtures
Effects of modified digestion schemes on the identification of proteins from complex mixtures
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DOI：
10.1021/pr050315j
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发表时间：
2006-03-01
影响因子：
4.4
通讯作者：
MacCoss, MJ
中科院分区：
文献类型：
--
作者：
Klammer, AA;MacCoss, MJ
在鸟枪法蛋白质组学中，复杂的蛋白质混合物被消化成肽，通过微毛细管液相色谱-串联质谱（LC-MS/MS）进行分离和鉴定。在该技术中，通常假设完全蛋白质消化。我们表明，相反，修改标准消化方案表现出大的，可重复的改进，在蛋白质鉴定，结果与消化是一个限制因素，在蛋白质鉴定的效率一致。
In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.