Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
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昆虫载体的凝溶胶负向调节植物呼肠孤病毒基于肌动蛋白的小管运动
DOI：
10.1186/s42483-019-0025-7
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Wei, Taiyun
中科院分区：
文献类型：
--
作者：
Chen, Qian;Zheng, Limin;Wei, Taiyun
大多数植物呼肠孤病毒编码一种非结构蛋白，其组装管状结构以包装病毒粒子用于在稻飞虱或叶蝉载体中的病毒传播。这些小管由肌动蛋白丝推动，有助于病毒克服昆虫媒介中的传播障碍。这被称为基于肌动蛋白的小管运动（ABTM），其中昆虫蛋白，特别是肌动蛋白相关蛋白参与其中。为了更好地了解昆虫在ABTM中的作用，研究了叶蝉载体中与水稻瘤矮病毒（RGDV）微管蛋白Pns11相互作用的蛋白质。我们发现凝溶胶蛋白，肌动蛋白调节蛋白，与Pns11在酵母双杂交系统和Sf9细胞相互作用。在叶蝉载体的培养细胞和昆虫体内也证实了凝溶胶蛋白和Pns11的相互作用和共定位。此外，在培养细胞和昆虫中，RGDV感染均上调凝溶胶蛋白的表达。通过RNA干扰触发的凝溶胶蛋白基因的敲低增加了病毒积累，从而增加了叶蝉载体的产毒率。凝溶胶蛋白与Pns11和病毒感染的这种负相关揭示了凝溶胶蛋白通过与Pns11小管相互作用而负面影响病毒传播的能力，最终起到负面调节RGDV感染的作用。本研究结果表明，在昆虫和病毒的协同进化过程中，昆虫对ABTM的表达具有负调控作用。
Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of theRice gall dwarf virus(RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.