Development of rapid oral bacteria detection apparatus based on dielectrophoretic impedance measurement method
Development of rapid oral bacteria detection apparatus based on dielectrophoretic impedance measurement method
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DOI：
10.1049/iet-nbt.2010.0011
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发表时间：
2011-06-01
影响因子：
2.3
通讯作者：
Konishi, K.
中科院分区：
文献类型：
--
作者：
Hamada, R.;Suehiro, J.;Konishi, K.
在本研究中，展示了一种基于介电泳阻抗测量（DEPIM）方法的细菌检测装置，用于快速评估口腔卫生。作者将微电极芯片（通过介电电泳 (DEP) 捕获细菌）、诱导 DEP 力的交流电压源以及阻抗测量电路集成到便携式仪器中，从而能够在医院和诊所进行快速、自动化的口腔细菌检查。特别考虑了口腔样品的高电导率对 DEP 力和 DEPIM 结果的影响。实验和理论上表明，使用较高的电场频率进行DEP细菌捕获和阻抗测量可以实现DEPIM应用于具有较高电导率的口腔样本的细菌检查。基于这些研究，作者优化了适合检查口腔样本的 DEPIM 频率条件，并设计和开发了用于口腔细菌现场检查的便携式 DEPIM 装置。在优化的频率条件下，DEPIM结果与传统培养方法非常吻合，表明DEPIM装置在实际快速口腔细菌检查中具有显着的适用性。
In this study, a bacteria detection apparatus based on dielectrophoretic impedance measurement (DEPIM) method was demonstrated for rapid evaluation of oral hygiene. The authors integrated a micro electrode chip on which bacteria were captured by dielectrophoresis (DEP), an AC voltage source to induce DEP force, and an impedance measurement circuit to a portable instrument that enables rapid and automated oral bacterial inspection in hospitals and clinics. Special considerations have been made on effects of high electrical conductivity of oral samples on DEP force and DEPIM results. It was shown experimentally and theoretically that using a higher electric field frequency for the DEP bacteria trap and the impedance measurement could realise DEPIM application to bacteria inspection from oral samples with higher conductivity. Based on these investigations, the authors optimised the frequency condition of the DEPIM suitable for inspecting an oral sample along with the design and development of a portable DEPIM apparatus for on-site inspection of oral bacteria. Under the optimised frequency condition, DEPIM results were in good agreement with the conventional culture method showing significant applicability of the DEPIM apparatus for practical rapid oral bacteria inspection.