Remote ischaemic preconditioning of the hind limb reduces experimental liver warm ischaemia-reperfusion injury
Remote ischaemic preconditioning of the hind limb reduces experimental liver warm ischaemia-reperfusion injury
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DOI：
10.1002/bjs.5331
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发表时间：
2006-06-01
影响因子：
9.6
通讯作者：
Davidson, B. R.
中科院分区：
文献类型：
--
作者：
Kanoria, S.;Jalan, R.;Davidson, B. R.
背景：肝脏直接缺血预处理可减少缺血再灌注损伤（IRI）。肢体远端缺血预处理（RIPC）已被证明可减少心脏IRI。本研究确定了短暂的远程缺血肢体在减少早期肝温IRI。方法：28只雄性家兔分为4组：假手术组，RIPC单独，IRI单独，RIPC + IRI。在肝脏IRI之前，用止血带在腿部诱导RIPC，通过10分钟缺血然后10分钟再灌注的三个交替循环。肝脏IRI产生的总流入闭塞25分钟。标记的肝损伤和全身和肝脏血流动力学测定2小时后reperfusion.Results：在2小时，IRI单独与血清转氨酶水平升高，平均动脉血压，肝血流量和外周血氧饱和度降低。有显着的改善，在这些变量的动物有RIPC前肝IRI，肝静脉硝酸盐/亚硝酸盐水平也显着higher.Conclusion：在这个实验模型RIPC出现，以减少肝脏IRI。
Background: Direct ischaemic preconditioning of the liver reduces ischaemia-reperfusion injury (IRI). Remote ischaemic preconditioning (RIPC) of a limb has been shown to reduce IRI to the heart. This study determined the effect of brief remote ischaemia to the limb in reducing early liver warm IRI.Methods: Twenty-eight male rabbits were allocated to four groups: sham operated, RIPC alone, IRI alone, and RIPC plus IRI. RIPC was induced in the leg with a tourniquet, before liver IRI, by three alternate cycles of 10 min ischaemia followed by 10 min reperfusion. Liver IRI was produced by total inflow occlusion for 25 min. Markers of liver injury and systemic and hepatic haemodynamics were measured for 2 h after reperfusion.Results: At 2 h, IRI alone was associated with increased serum levels of aminotransferases, and reduced mean arterial blood pressure, hepatic blood flow and peripheral oxygen saturation. There was significant improvement in these variables in animals that had RIPC before liver IRI, and hepatic venous nitrate/nitrite levels were also significantly higher.Conclusion: In this experimental model RIPC appeared to reduce liver IRI.