Evidence for a positive regulatory role of strawberry (Fragariaxananassa) Fa WRKY1 and Arabidopsis At WRKY75 proteins in resistance
Evidence for a positive regulatory role of strawberry (Fragariaxananassa) Fa WRKY1 and Arabidopsis At WRKY75 proteins in resistance
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DOI：
10.1093/jxb/erp152
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发表时间：
2009-07-01
影响因子：
6.9
通讯作者：
Caballero, Jose L.
中科院分区：
文献类型：
--
作者：
Encinas-Villarejo, Sonia;Maldonado, Ana M.;Caballero, Jose L.
关于除拟南芥以外的物种中植物对病原体抗性的分子基础的知识有限。本研究对从重要果树草莓（Fragariaxananassa）中分离到的第一个WRKY基因Fa WRKY 1的功能进行了研究。Fa WRKY 1编码IIc WRKY转录因子，并且在刺盘孢（Colletotrichum acutatum）感染、用激发子处理和创伤后在草莓中上调。其拟南芥序列同源物，在WRKY 75，已被描述为在调节磷酸盐饥饿反应中发挥作用。然而，使用T-DNA插入突变体，在拟南芥中的基础和R-介导的抗性的激活中的作用的At WRKY 75和Fa WRKY 1被证明。在wrky 75突变体更容易受到有毒和无毒的假单胞菌菌株。Fa WRKY 1在At wrky 75突变体和野生型中的过表达逆转了突变体的增强的易感表型，并且甚至增加了对P.耐药表型与致病相关（PR）基因表达不相关，但与强氧化爆发和谷胱甘肽-S-转移酶（GST）诱导相关。综上所述，这些结果表明，在拟南芥中，At WRKY 75和Fa WRKY 1在相容性和不相容性相互作用期间充当防御的正调节因子，并且Fa WRKY 1很可能是介导对C.草莓中的尖叶草此外，这些结果提供了证据，拟南芥可以是一个有用的模式，在酒渣鼻物种的功能研究，如草莓。
Knowledge of the molecular basis of plant resistance to pathogens in species other than Arabidopsis is limited. The function of Fa WRKY1, the first WRKY gene isolated from strawberry (Fragariaxananassa), an important agronomical fruit crop, has been investigated here. Fa WRKY1 encodes a IIc WRKY transcription factor and is up-regulated in strawberry following Colletotrichum acutatum infection, treatments with elicitors, and wounding. Its Arabidopsis sequence homologue, At WRKY75, has been described as playing a role in regulating phosphate starvation responses. However, using T-DNA insertion mutants, a role for the At WRKY75 and Fa WRKY1 in the activation of basal and R-mediated resistance in Arabidopsis is demonstrated. At wrky75 mutants are more susceptible to virulent and avirulent isolates of Pseudomonas syringae. Overexpression of Fa WRKY1 in At wrky75 mutant and wild type reverts the enhanced susceptible phenotype of the mutant, and even increases resistance to avirulent strains of P. syringae. The resistance phenotype is uncoupled to PATHOGENESIS-RELATED (PR) gene expression, but it is associated with a strong oxidative burst and glutathione-S-transferase (GST) induction. Taken together, these results indicate that At WRKY75 and Fa WRKY1 act as positive regulators of defence during compatible and incompatible interactions in Arabidopsis and, very likely, Fa WRKY1 is an important element mediating defence responses to C. acutatum in strawberry. Moreover, these results provide evidence that Arabidopsis can be a useful model for functional studies in Rosacea species like strawberry.