Characterization and Genomic Analysis of a Highly Efficient Dibutyl Phthalate-Degrading Bacterium Gordonia sp. Strain QH-12.
Characterization and Genomic Analysis of a Highly Efficient Dibutyl Phthalate-Degrading Bacterium Gordonia sp. Strain QH-12.
复制标题
高效邻苯二甲酸二丁酯降解细菌 Gordonia sp. 的表征和基因组分析。
DOI：
10.3390/ijms17071012
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发表时间：
2016-06-25
影响因子：
5.6
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Jin D;Kong X;Liu H;Wang X;Deng Y;Jia M;Yu X
根据16S rRNA基因序列分析，从活性污泥中分离出的菌株QH-12为Gordonia sp.，该菌株能够利用邻苯二甲酸二丁酯（DBP）和其他常见的邻苯二甲酸酯（PAEs）作为唯一的碳和能量来源。菌株QH-12在不同浓度下对DBP的降解动力学符合修正的Gompertz模型（R2 > 0.98）。而菌株QH-12不能利用主要中间产物邻苯二甲酸酯（phthalic acid； PA）作为唯一的碳源和能量来源，仅检测到少量的PA。QH-12基因组分析显示，存在与PAEs降解有关的水解酶/酯酶基因，但未发现邻苯二甲酸分解代谢基因簇，表明在Gordonia sp. QH-12中存在一种新的PAEs降解途径。这些信息将有助于更全面地了解降解paes的戈登菌的不同遗传机制。
A bacterial strain QH-12 isolated from activated sludge was identified as Gordonia sp. based on analysis of 16S rRNA gene sequence and was found to be capable of utilizing dibutyl phthalate (DBP) and other common phthalate esters (PAEs) as the sole carbon and energy source. The degradation kinetics of DBP under different concentrations by the strain QH-12 fit well with the modified Gompertz model (R2 > 0.98). However, strain QH-12 could not utilize the major intermediate product phthalate (phthalic acid; PA) as the sole carbon and energy source, and only a little amount of PA was detected. The QH-12 genome analysis revealed the presence of putative hydrolase/esterase genes involved in PAEs-degradation but no phthalic acid catabolic gene cluster was found, suggesting that a novel degradation pathway of PAEs was present in Gordonia sp. QH-12. This information will be valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs-degrading Gordonia sp. strains.