Sulindac sulfide inhibits the proliferation of colon cancer cells: Diminished expression of the proliferation markers PCNA and Ki-67
Sulindac sulfide inhibits the proliferation of colon cancer cells: Diminished expression of the proliferation markers PCNA and Ki-67
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DOI：
10.1016/s0304-3835(97)04740-x
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发表时间：
1997-05-19
期刊：
影响因子：
9.7
通讯作者：
Rigas, B
中科院分区：
文献类型：
--
作者：
Qiao, L;Shiff, SJ;Rigas, B
我们评估了舒林酸硫化物（SS），减少细胞数量和诱导细胞凋亡培养的结肠癌细胞（CCCs），增殖标志物PCNA和Ki-67的表达在HT-29和HCT-15 CCCs的影响;只有前者表达环加氧酶。双变量流式细胞术分析DNA含量和PCNA/Ki-67表达。SS抑制细胞增殖，通过减少PCNA和Ki-67的表达来确定，在72 h时大约减少一半，并诱导细胞凋亡（分别占细胞数量减少的约三分之二和三分之一）。SS在HT-29和HCT-15 CCC中以及在非结肠细胞中也发生了类似的作用，表明SS对培养细胞的这种相当普遍的作用不依赖于前列腺素合成的抑制。(C)1997 Elsevier Science爱尔兰有限公司
We evaluated the effect of sulindac sulfide (SS), which reduces cell number and induces apoptosis in cultured colon cancer cells (CCCs), on expression of the proliferation markers PCNA and Ki-67 in HT-29 and HCT-15 CCCs; only the former express cyclooxygenases. DNA content and PCNA/Ki-67 expression were analyzed by bivariate flow cytometry. SS inhibited cell proliferation, determined by the reduced expression of PCNA and Ki-67, roughly by half at 72 h, and induced apoptosis (accounting for about two-thirds and one-third of the reduction in cell number, respectively). A similar effect of SS occurred in HT-29 and HCT-15 CCCs, and also in non-colonic cells, indicating that this rather general effect of SS on cultured cells is not dependent on inhibition of prostaglandin synthesis. (C) 1997 Elsevier Science Ireland Ltd.