Photoelectrochemical platform for MicroRNA let-7a detection based on graphdiyne loaded with AuNPs modified electrode coupled with alkaline phosphatase
Photoelectrochemical platform for MicroRNA let-7a detection based on graphdiyne loaded with AuNPs modified electrode coupled with alkaline phosphatase
复制标题
基于负载AuNPs修饰电极的石墨二炔与碱性磷酸酶耦合的MicroRNA let-7a检测的光电化学平台
DOI：
10.1016/j.bios.2019.02.002
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发表时间：
2019-04-01
影响因子：
12.6
通讯作者：
Hun, Xu
中科院分区：
文献类型：
--
作者：
Li, Yanxin;Li, Xiaohua;Hun, Xu
本工作成功地合成了一种负载金纳米粒子的光活性材料石墨二炔（GDY）（AuNPs-GDY）。所制备的材料利用了GDY的自然带隙结构，其可以产生空穴-电子对和AuNPs的等离子体共振效应，以获得高的光电化学（PEC）响应。AuNPs-GDY PEC响应随GDY与四氯金酸的质量比而变化。当GDY与四氯金酸的质量比为1：2.5时，AuNPs-GDY具有最佳的PEC性能。因此，选择最佳的一个作为光活性材料，建立PEC生物传感器的microRNA检测。PEC生物传感器使用碱性磷酸酶作为催化剂，原位产生抗坏血酸，这提供了低背景信号和高PEC响应。选择癌症标志物MicroRNA let-7a作为靶模型。在pH8.0、电位300 mV的最佳条件下，PEC生物传感器的检测限为13 × 10 ~（-19）M，对microRNA let-7a的线性范围为1.0 × 10 ~（-18）M ~ 1.0 × 10 ~（-1）M。这种PEC生物传感器为利用GDY构建分析方法和检测超低水平的microRNA进行诊断提供了一个有前途的平台。
In this work, a photoactive material, Graphdiyne (GDY) loaded with AuNPs (AuNPs-GDY), was successfully synthesized. The fabricated material made use of the natural band-gap structure of GDY, which could produce hole-electron pairs and the plasmon resonance effect of AuNPs to obtain a high photoelectrochemical (PEC) response. AuNPs-GDY PEC response changed with the mass ratio of GDY to tetrachloroauric acid. When the mass ratio of GDY to tetrachloroauric acid was 1:2.5, AuNPs-GDY exhibited the best PEC performance. Thus, the best one was selected as the photoactive material to establish a PEC biosensor for microRNA detection. The PEC biosensor used the alkaline phosphatases as catalyzer to generate ascorbic acid in situ, which provided a low background signal and a high PEC response. The cancer marker, MicroRNA let-7a, was chosen as a target model. Under optimal condition, potential 300 mV and pH 8.0, the PEC biosensor had a detection limit of 13 x 10(-19) M and a good linearity with microRNA let-7a concentration ranged from 1.0 x 10(-18) M to 1.0 x 10(-1) M. This PEC biosensor opened a promising platform using GDY to fabricate analytical method and detect microRNA at ultralow levels for diagnoses.