Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients
Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients
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DOI：
10.1111/j.1399-302x.2007.00354.x
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发表时间：
2007-06-01
影响因子：
--
通讯作者：
Ishikawa, I.
中科院分区：
文献类型：
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作者：
Thiha, K.;Takeuchi, Y.;Ishikawa, I.
简介：识别组织中入侵的牙周病细菌对于确定它们在牙周病发病机制中的作用非常重要。本研究的目的是鉴定牙周炎患者患病牙龈组织中的牙周病细菌。方法：收集 32 例广泛性慢性牙周炎（CP）、16 例广泛性侵袭性牙周炎（GAgP）和 8 例局部侵袭性牙周炎（LAgP）患者的龈下菌斑和牙龈组织。采用聚合酶链式反应研究了牙龈卟啉单胞菌、伴放线放线杆菌和福赛坦纳菌的检测频率和数量。还检测了口腔链球菌和远缘链球菌的流行情况，并观察了伴放线菌血清型的分布。结果：在三个牙周炎组中，约70%的组织样本和50%的牙菌斑样本中检测到牙龈卟啉单胞菌和福赛菌。 LAgP 组 (63%) 组织样本中 A. actinomycetemcomitans 的患病率高于 CP (16%) 或 GAgP (38%) 组。在 50% 的 LAgP 患者中检测到 C 型放线菌。在所有三个牙周炎组的牙菌斑和组织样本中，口腔链球菌和远缘链球菌的检测频率均明显较低。 3个牙周炎组组织样本中P. gingivalis、A. actinomycetemcomitans和T. forsythensis的含量无差异。结论：P. gingivalis、A. actinomycetemcomitans和T. forsythensis可定位于病变牙龈组织，可能参与牙周组织破坏。 c 血清型是日本 LAgP 患者中 A. actinomycetemcomitans 的主要血清型。
Introduction: The identification of invading periodontopathic bacteria in tissues is important to determine their role in the pathogenesis of periodontal disease. The objective of this study was to identify periodontopathic bacteria in diseased gingival tissue of periodontitis patients.Methods: Subgingival plaque and gingival tissue were collected from 32 generalized chronic periodontitis (CP), 16 generalized aggressive periodontitis (GAgP) and eight localized aggressive periodontitis (LAgP) patients. Detection frequencies and quantities of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Tannerella forsythensis were investigated by polymerase chain reaction. The prevalences of Streptococcus oralis and Streptococcus sobrinus were also examined and the distribution of A. actinomycetemcomitans serotypes was observed.Results: P. gingivalis and T. forsythensis were detected in approximately 70% of tissue samples and 50% of plaque samples in the three periodontitis groups. Prevalence of A. actinomycetemcomitans in tissue samples was higher in the LAgP (63%) group than in either the CP (16%) or the GAgP (38%) group. A. actinomycetemcomitans serotype c was detected in 50% of LAgP patients. Detection frequencies of S. oralis and S. sobrinus were markedly low in both plaque and tissue samples from all three periodontitis groups. Amounts of P. gingivalis, A. actinomycetemcomitans and T. forsythensis in the tissue samples were not different among the three periodontitis groups.Conclusion: P. gingivalis, A. actinomycetemcomitans and T. forsythensis can localize in diseased gingival tissue and may be involved in periodontal tissue destruction. Serotype c is the predominant serotype of A. actinomycetemcomitans in Japanese LAgP patients.