Lysosomal enzymes in the acrosome and their possible role in fertilization.
Lysosomal enzymes in the acrosome and their possible role in fertilization.
复制标题
顶体中的溶酶体酶及其在受精中的可能作用。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
E. Hartree
中科院分区：
文献类型：
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作者：
A. Allison;E. Hartree
摘要公羊精子用低渗tris-HCl缓冲液洗涤，获得几乎不含精浆和细胞质液滴的精子。用阳离子洗涤剂（Hyamine 2389）孵育精子，使顶体脱落。检查顶体制备物和缓冲液洗涤液中的以下溶酶体酶：酸性磷酸酶、芳基硫酸酯酶、I g=B I-N-乙酰氨基葡糖苷酶、磷脂酶A和蛋白酶。所有的酶活性检测，无论是在洗涤液和顶体制剂。后者的放射性水平比假设所有活动都是由于洗涤物的污染而预期的要高得多。蛋白酶活性在pH7.0时最高。5.用Euchrysine 3R活体染色后，羊、牛、猪和人精子顶体荧光不明显。然而，豚鼠、仓鼠和几种啮齿类动物的顶体显示溶酶体典型的明亮的橙红色荧光。当精子细胞成熟时，高尔基带周围的红色荧光颗粒凝聚形成红色荧光前顶体。酸性磷酸酶在颗粒、顶体、顶体和通过高速离心顶体制备物获得的颗粒中进行组织化学检测。在所有发育阶段，溴氯吲哚乙酸酯酶的组织化学测试表明，这种酶只存在于顶体。有证据表明，顶体是一种专门的溶酶体，在多细胞生物中进化以促进受精。
Summary. Ram spermatozoa were washed with hypotonic tris-HCl buffer and obtained almost free of seminal plasma and cytoplasmic droplets. Acrosomes were dislodged by incubating the spermatozoa with a cationic detergent (Hyamine 2389). The acrosomal preparation and the buffer washings were examined for the following lysosomal enzymes: acid phosphatase, aryl sulphatase, \g=b\-N-acetylglucosaminidase, phospholipase A and proteases. All enzyme activities were detected, both in washings and in acrosomal preparations. The levels of activity in the latter were much higher than could be expected on the assumption that all activities were due to contamination by washings. Protease activity was greatest at pH 7\m=.\5. After vital staining with Euchrysine 3R, acrosomal fluorescence in ram, bull, boar and human spermatozoa is not very conspicuous. However, acrosomes of guinea-pig, hamster and several rodents show the brilliant orange-red fluorescence typical of lysosomes. As spermatids mature, red-fluorescing granules around the Golgi zone condense to form the red-fluorescent pro-acrosomes. Acid phosphatase was detectable histochemically in granules, pro\x=req-\ acrosomes, acrosomes and the pellets obtained by high-speed centrifugation of acrosomal preparations. At all developmental stages, histochemical tests for bromochloroindoxyl acetate esterase showed that this enzyme was present only in the acrosome. The evidence suggests that the acrosome is a specialized lysosome which evolved to facilitate fertilization in multicellular organisms.