In situ studies of the distal convoluted tubule in the rat. I. Evidence for NaCl secretion.
In situ studies of the distal convoluted tubule in the rat. I. Evidence for NaCl secretion.
复制标题
大鼠远曲小管的原位研究。
DOI：
10.1152/ajprenal.1982.243.2.f160
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
G. Schubert
中科院分区：
文献类型：
--
作者：
J. Schnermann;J. Briggs;G. Schubert
METHODSExperiments were performed on male Munich-Wistar rats ranging in weight from 95 to 140 g. The animals were anesthetized with an intraperitoneal injection of 110 mg/kg Inactin and placed on a heated operating table. After a catheter was inserted into the jugular vein a 4% bovine serum albumin solution in isotonic saline was infused at 0.9 ml/h for 1 h; during the rest of the experiment isotonic saline containing 1% bovine serum albumin was infused at the same rate. Earlier experiments have shown that this protocol attenuates the rise in hematocrit usually seen following laparotomy and maintains it close to normal during the course of the experiment. The femoral artery was cannulated for measurement of arterial blood pressure. The left kidney was prepared for micropuncture through a left flank incision.
