Diffusion-based analysis of molecular interactions in microfluidic devices
Diffusion-based analysis of molecular interactions in microfluidic devices
复制标题
DOI：
10.1109/jproc.2003.820547
复制
发表时间：
2004-01-01
影响因子：
20.6
通讯作者：
Yager, P
中科院分区：
文献类型：
--
作者：
Hatch, A;Garcia, E;Yager, P
生物流体中分子相互作用的研究作为阐明分子和生物学功能的研究工具是重要的，用于发现或设计具有期望功能的分子，以及用于测量或检测用于临床诊断目的的分析物。微流体技术是一种新兴技术，已被证明可用于研究和控制分子相互作用，具有减少样品和试剂体积、缩短反应时间、便携式仪器和高通量的潜在优势。在微尺度尺寸下，许多生物相关分子的扩散运输可以在短时间内（几秒到几分钟）覆盖流体通道的大部分。我们已经利用这一功能来分析分子间的相互作用的基础上的变化，在扩散运输的反应成分之一。在这里，我们提出了一个新的分析配置研究分子结合的相互作用，并报告在制造和使用的水凝胶扩散为基础的分析的新发展。我们还概述了系统配置和分析技术，已被证明是有用的研究生物分析物的分子相互作用，并讨论了他们的能力，使用复杂的流体，如血液。
The study of molecular interactions in biological fluids is important as a research tool to elucidate molecular and biological function, for discovering or designing molecules that have a desirable function, and for measuring or detecting analytes for clinical diagnostic purposes. Microfluidics is an emerging technology that has proven useful for studying and controlling molecular interactions with the potential advantages of reduced sample and reagent volumes, short reaction times, portable instrumentation, and high throughput. At microscale dimensions, diffusive transport of many biologically relevant molecules can cover large fractions of a fluid channel in a short time (seconds to minutes). We have exploited this feature to analyze molecular interactions based on changes in the diffusive transport of one of the reacting components. Here, we present a new assay configuration for studying molecular binding interactions and report new developments in the fabrication and use of hydrogels for diffusion-based analysis. We also overview system configurations and analysis techniques that have proven useful for studying molecular interactions of biological analytes and discuss their ability to operate using complex fluids such as blood.