Wound-induced RNaseLE expression is jasmonate and systemin independent and occurs only locally in tomato (Lycopersicon esculentum cv. Lukullus)
Wound-induced RNaseLE expression is jasmonate and systemin independent and occurs only locally in tomato (Lycopersicon esculentum cv. Lukullus)
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DOI：
10.1016/j.phytochem.2004.04.036
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发表时间：
2004-05-01
期刊：
影响因子：
3.8
通讯作者：
Köck, M
中科院分区：
文献类型：
--
作者：
Gross, N;Wasternack, C;Köck, M
番茄RiNaseLE由磷酸盐缺乏和创伤诱导，并且可能在大分子再循环以及创伤愈合中发挥作用。在此，我们分析了茉莉酸和系统素在创伤诱导的RNaseLE激活中的作用。RNaseLE的快速表达后，受伤的叶子导致最大的RNase活性在10小时内，出现只有局部。茉莉酸（JA）或其分子模拟物乙基茚满酰异亮氨酸缀合物不诱导RNaseLE表达。相应地，RNaseLE表达创伤后的35 S：：丙二烯氧化物环化酶反义植物已知是JA缺陷。RNaseLE在系统素处理后不表达，但在受系统素感知影响的spr1突变体中局部表达。在携带PromLE：：uidA构建体的番茄植物中，可在创伤后检测到GUS活性。但在用JA或系统素处理后不发生。这些数据表明RNaseLE表达的局部作用的创伤诱导型系统素和JA非依赖性信号传导途径。(C)2004爱思唯尔有限公司保留所有权利。
Tomato RiNaseLE is induced by phosphate deficiency and wounding and may play a role in macromolecular recycling as well as wound healing. Here, we analyzed the role of jasmonate and systemin in the wound-induced RNaseLE activation. The rapid expression of RNaseLE upon wounding of leaves leading to maximal RNase activity within 10 h, appeared only locally. Jasmonic acid (JA) or its molecular mimic ethyl indanoyl isoleucine conjugate did not induce RNaseLE expression. Correspondingly, RNaseLE was expressed upon wounding of 35S::allene oxide cyclase antisense plants known to be JA deficient. RNaseLE was not expressed upon systemin treatment, but was locally expressed in the spr1 mutant which is affected in systemin perception. In tomato plants carrying a PromLE::uidA construct, GUS activity could be detected upon wounding., but not following treatment with JA or systemin. The data indicate a locally acting wound-inducible systemin- and JA-independent signaling pathway for RNaseLE expression. (C) 2004 Elsevier Ltd. All rights reserved.