A developmentally regulated neuraminidase activity in Trypanosoma cruzi.
A developmentally regulated neuraminidase activity in Trypanosoma cruzi.
复制标题
克氏锥虫中发育调节的神经氨酸酶活性。
DOI：
10.1126/science.6338592
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Pereira,ME
中科院分区：
文献类型：
--
作者：
Pereira,ME
人类病原体克氏锥虫（Y株）含有一种神经氨酸酶活性，在寄生虫的不同发育阶段变化很大。从组织培养和感染小鼠的血液中获得的感染性锥乳鞭毛虫的特异性神经氨酸酶活性比非细胞培养形式高7至15倍。无尾线虫缺乏酶活性。该酶的最适apH为6.0 ~ 6.5。活锥虫从人红细胞和血浆糖蛋白中释放唾液酸。几种唾液酰类化合物被寄生虫水解，但最好的底物是蛋白质或类体。t病毒感染小鼠的红细胞。花生凝集素对寄生虫有凝集作用，凝集效价与寄生虫病程度相关。
The human pathogenTrypanosoma cruzi(Y strain) contains a neuraminidase activity that varies widely in the different developmental stages of the parasite. The specific neuraminidase activity of infective trypomastigotes obtained from tissue culture and from the bloodstream of infected mice is 7 to 15 times higher than that of the acellular culture forms. Amastigotes were devoid of enzyme activity. The enzyme has apH optimum of 6.0 to 6.5. Live trypanosomes released sialic acid from human erythrocytes and plasma glycoproteins. Several sialyl compounds were hydrolyzed by the parasite, but the best substrate was the protein orosomucoid. Erythrocytes from infected mice withT. cruziparasitemia were agglutinated by peanut lectin and the hemagglutination titer was correlated with the degree of parasitemia.