CLONING AND CHARACTERIZATION OF HUMAN AND MOUSE HOMOLOGS OF THE DROSOPHILA CALCIUM-ACTIVATED POTASSIUM CHANNEL GENE, SLOWPOKE
CLONING AND CHARACTERIZATION OF HUMAN AND MOUSE HOMOLOGS OF THE DROSOPHILA CALCIUM-ACTIVATED POTASSIUM CHANNEL GENE, SLOWPOKE
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DOI：
10.1093/hmg/3.8.1239
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发表时间：
1994-08-01
影响因子：
3.5
通讯作者：
GANETZKY, B
中科院分区：
文献类型：
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作者：
PALLANCK, L;GANETZKY, B
钾通道在多种生理过程中发挥着重要作用。尽管已经在分子水平上分析了编码电压激活钾通道的几种基因，但尚未在人类中表征钙激活钾通道基因。为了为此类多肽的功能分析提供基础，我们报道了果蝇钙激活钾通道基因slowpoke的小鼠和人类同源物的克隆。人类和小鼠基因编码的多肽与果蝇对应物具有超过 50% 的氨基酸同一性。此外，与果蝇slowpoke基因一样，小鼠和人类基因都通过选择性剪接产生多个转录本。根据对人仓鼠杂交细胞系基因组 DNA 进行聚合酶链式反应分析的结果，将人类基因映射到 10 号染色体。由于钙激活钾通道参与多种细胞功能，包括神经肌肉通讯、分泌和细胞免疫，因此对其的持续分析有望具有广泛的生物学和医学意义。
Potassium channels play important roles in a wide variety of physiological processes. Although several genes encoding voltage-activated potassium channels have been analyzed at the molecular level, no calcium-activated potassium channel gene has yet been characterized in humans. In an effort to provide the foundation for functional analysis of such polypeptides we report the cloning of mouse and human homologs of the Drosophila melanogaster calcium-activated potassium channel gene, slowpoke. Both the human and mouse genes encode polypeptides that have more than 50% amino acid identities with their Drosophila counterpart. In addition, like the Drosophila slowpoke gene, both the mouse and human genes generate multiple transcripts by alternative splicing. The human gene maps to chromosome 10 based on the results of polymerase chain reaction analysis of genomic DNA from human-hamster hybrid cell lines. Because calcium-activated potassium channels participate in wide variety of cellular functions including neuromuscular communication, secretion and cellular immunity, their continued analysis promises to have broad biological and medical significance.