Amplification of 5' end cDNA with 'new RACE'.
Amplification of 5' end cDNA with 'new RACE'.
复制标题
使用“new RACE”扩增 5 端 cDNA。
DOI：
10.1038/nprot.2006.479
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发表时间：
2006
期刊：
影响因子：
14.8
通讯作者：
Frohman,MichaelA
中科院分区：
文献类型：
--
作者：
Scotto-Lavino,Elizabeth;Du,Guangwei;Frohman,MichaelA
“New RACE”（cDNA末端快速扩增）PCR是一种获取仅部分序列已知的mRNA全长cDNA的方法。从细胞mRNA开始，PCR用于扩增序列的已知部分和cDNA末端的非特异性标签之间的区域。在“new RACE”中，在逆转录之前，锚点连接到mRNA的5 ‘端，导致选择性地产生全长5 ’ cDNA末端。虽然‘new RACE’也可以用于扩增3 ‘端，但这里只介绍了获得5 ’端的协议。该方案可在1-3天内完成。
'New RACE' (rapid amplification of cDNA ends) PCR is a method for obtaining full-length cDNA for mRNA for which only part of the sequence is known. Starting with cellular mRNA, PCR is used to amplify regions between the known parts of the sequence and nonspecific tags at the ends of the cDNA. In 'new RACE', an anchor is ligated to the 5′ end of the mRNA before reverse transcription, resulting in the selective production of full-length 5′ cDNA ends. Although 'new RACE' can also be used to amplify 3′ ends, only the protocol for obtaining 5′ ends is presented here. This protocol can be completed in 1–3 days.