Antigenic variation of envelope and internal proteins of mumps virus strains detected with monoclonal antibodies.
Antigenic variation of envelope and internal proteins of mumps virus strains detected with monoclonal antibodies.
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用单克隆抗体检测腮腺炎病毒株包膜和内部蛋白的抗原变异。
DOI：
10.1099/0022-1317-67-2-281
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
E. Norrby
中科院分区：
文献类型：
--
作者：
R. Rydbeck;A. Löve;C. Orvell;E. Norrby
用免疫荧光和放射免疫沉淀法（RIPA）测定了9株腮腺炎病毒的抗原特性。这些抗体针对腮腺炎病毒的五种不同结构组分，血凝素-神经氨酸酶（HN）、融合（F）、基质（M）、磷酸（P）和核衣壳（NP）蛋白。9株流行性腮腺炎病毒根据其抗原特性可分为两类。一组包括十多年前分离的两种菌株和Jeryl林恩疫苗株。这三个菌株反应范围更广的单克隆抗体比第二组的6个最近分离的菌株不同的地理来源。在F、M和P蛋白中，仅在单个抗原决定簇中发现变异。在HN和NP组分中，RIPA分别揭示了三个和七个决定因素的变化。Jeryl林恩疫苗株显示与RIPA中的一个抗HN抗体克隆无反应。
Antigenic characteristics of nine mumps virus strains were determined by immunofluorescence and radioimmunoprecipitation assay (RIPA) using a collection of 44 monoclonal antibodies. These antibodies were directed against five different structural components of mumps virus, the haemagglutinin-neuraminidase (HN), fusion (F), matrix (M), phospho- (P) and nucleocapsid (NP) proteins. The nine mumps virus strains could be divided into two groups according to their antigenic characteristics. One group included two strains isolated more than a decade ago and the Jeryl Lynn vaccine strain. These three strains reacted with a wider range of monoclonal antibodies than the second group of six recently isolated strains of different geographical origin. In the F, M and P proteins variations were only found in single antigenic determinants. In the HN and NP components, RIPA revealed variations in three and seven determinants respectively. The Jeryl Lynn vaccine strain showed a unique lack of reaction with one anti-HN antibody clone in the RIPA.
通过单克隆抗体对病毒进行抗原表征。
影响因子：
10.5
作者：
Yewdell,JW;Gerhard,W
通讯作者：
Gerhard,W