Ascorbic acid and colon cancer: an oxidative stimulus to cell death depending on cell profile
Ascorbic acid and colon cancer: an oxidative stimulus to cell death depending on cell profile
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DOI：
10.1016/j.ejcb.2016.04.001
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发表时间：
2016-06-01
影响因子：
6.6
通讯作者：
Botelho, Maria Filomena
中科院分区：
文献类型：
--
作者：
Pires, Ana Salome;Marques, Claudia Raquel;Botelho, Maria Filomena
结直肠癌是全球范围内的主要健康问题，迫切需要新的有效的抗癌方法，以允许治疗，提高生存率和改善患者的生活质量。在药理学浓度下，抗坏血酸（AA）具有选择性的细胞毒作用，其细胞毒作用机制尚未解决。有人认为，这取决于生产的细胞外过氧化氢，使用抗坏血酸自由基作为中间体。本研究的目的是评估AA在三种结肠癌细胞系中诱导的作用，以及可能的细胞死亡机制，我们的结果表明，药理浓度的AA诱导的抗增殖，细胞毒性和遗传毒性作用在研究中的三种结肠癌细胞系。我们还发现，AA可以通过增加氧化应激诱导细胞死亡，但也介导了ROS非依赖性机制，如在LS 1034细胞中观察到的。这项工作探讨了AA的抗肿瘤作用，并强调了其在CC治疗中的适用性，强调了进行临床试验的重要性。(C)2016 Elsevier GmbH. All rights reserved.
Colorectal cancer is a major health problem worldwide with urgent need for new and effective anti-cancer approaches that allow treating, increasing survival and improving life quality of patients. At pharmacological concentrations, ascorbic acid (AA) exerts a selective cytotoxic effect, whose mechanism of cytotoxicity remains unsolved. It has been suggested that it depends on the production of extracellular hydrogen peroxide, using ascorbate radical as an intermediate. The aim of this study was to evaluate the effects induced by AA in three colon cancer cell lines, as well as, possible cell death mechanisms involved.Our results showed that pharmacological concentrations of AA induce anti-proliferative, cytotoxic and genotoxic effects on three colon cancer cell lines under study. We also found that AA can induce cell death by an increment of oxidative stress, but also mediating a ROS-independent mechanism, as observed in LS1034 cells. This work explores AA anti-tumoral effects and highlights its applicability in the treatment of CC, underlying the importance of proceeding to clinical trials. (C) 2016 Elsevier GmbH. All rights reserved.