The Bombyx mori karyotype and the assignment of linkage groups
The Bombyx mori karyotype and the assignment of linkage groups
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DOI：
10.1534/genetics.104.040352
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发表时间：
2005-06-01
期刊：
影响因子：
3.3
通讯作者：
Sahara, K
中科院分区：
文献类型：
--
作者：
Yoshido, A;Bando, H;Sahara, K
鳞翅目昆虫有较多的小的全着丝粒染色体(家蚕，2n=56)。长期以来，染色体鉴定在这个群体中一直受到数量较多和缺乏合适的标记，如着丝粒位置和染色体条带的阻碍。本研究以遗传定位的家蚕细菌人工染色体(BACs)为探针，对减数分裂染色体互补进行了荧光原位杂交(FISH)。每条染色体上有两到四个绿色或红色荧光标记的探针的组合，使我们能够根据其标记模式明确地识别家蚕核型的28个二价体。每条染色体被分配给一个已经建立的遗传连锁组，并确定了染色体的正确方向。这有助于对任何其他序列的物理作图，并与正在进行的家蚕基因组计划相关。双色BAC-FISH核型分析克服了常规显带技术不可用的生物中的染色体识别问题。
Lepidopteran species have a relatively high number of small holocentric chromosomes (Bombyx mori, 2n = 56). Chromosome identification has long been hampered in this group by the high number and by the absence of suitable markers like centromere position and chromosome bands. In this study, we carried out fluorescence in situ hybridization (FISH) on meiotic chromosome complements using genetically mapped B. mori bacterial artificial chromosomes (BACs) as probes. The combination of two to four either green or red fluorescence-labeled probes per chromosome allowed us to recognize unequivocally each of the 28 bivalents of the B. mori karyotype by its labeling pattern. Each chromosome was assigned one of the already established genetic linkage groups and the correct orientation in the chromosome was defined. This facilitates physical mapping of any other sequence and bears relevance for the ongoing B. mori genome projects. Two-color BAC-FISH karyotyping overcomes the problem of chromosome recognition in organisms where conventional banding techniques are not available.